Use of order-specific primers to investigate the methanogenic diversity in acetate enrichment system.

Journal of Industrial Microbiology & Biotechnology
Seung Gu ShinSeokhwan Hwang

Abstract

The applicability of order-specific primers in minimizing the possible underestimation of microbial diversity was evaluated via denaturing gradient gel electrophoresis (DGGE) analysis of a lab-scale anaerobic digester. Initially, a population analysis with real-time quantitative PCR demonstrated the existence of three methanogenic orders--Methanobacteriales, Methanomicrobiales, and Methanosarcinales--throughout the reaction period. DGGE analyses with three pairs of order-specific primers yielded eight operational taxonomic units (OTUs), whereas DGGE analysis with two independent Archaea-specific primers identified only five. Moreover, the order-specific primers amplified at least one OTU affiliated with each order, whereas no members of Methanobacteriales or Methanomicrobiales were identified with Archaea-specific primers in most samples. These findings provide evidence that order-specific analysis can detect the diversity of methanogens in greater detail than conventional Archaea-specific analysis.

References

Aug 10, 2000·Analytical Biochemistry·S AyyadevaraR J Shmookler Reis
Sep 21, 2001·Journal of the American Animal Hospital Association·G M Griffin, D E Holt
Jan 5, 2002·Applied Microbiology and Biotechnology·Y UenoY Igarashi
Aug 22, 2002·Water Research·Wen-Tso LiuHerbert H P Fang
Nov 11, 2003·Journal of Microbiological Methods·G C BakerD A Cowan
Dec 4, 2003·Environmental Microbiology·Pierre E GalandKim Yrjälä
Jun 16, 2004·Current Opinion in Microbiology·Larry J ForneyCeleste J Brown
Jan 11, 2005·Applied and Environmental Microbiology·Dimitar KarakashevIrini Angelidaki
Apr 16, 2005·Systematic and Applied Microbiology·Kees RoestAntoon D L Akkermans
Jun 11, 2005·Environmental Microbiology·Natasha BanningAndrew J Weightman
Sep 5, 2006·Applied and Environmental Microbiology·Eitan Ben-DovAriel Kushmaro
Feb 27, 2007·Trends in Microbiology·Kerry S Smith, Cheryl Ingram-Smith

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Citations

Jul 9, 2013·Journal of Industrial Microbiology & Biotechnology·Kyungjin ChoSeokhwan Hwang
Apr 5, 2011·Applied and Environmental Microbiology·Sabrina BeckmannHeribert Cypionka
Jan 1, 2012·ISRN Microbiology·Joshua T EllisCharles D Miller

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