Use of photolithography to encode cell adhesive domains into protein microarrays

Langmuir : the ACS Journal of Surfaces and Colloids
Ji Youn LeeAlexander Revzin

Abstract

Protein microarrays are rapidly emerging as valuable tools in creating combinatorial cell culture systems where inducers of cellular differentiation can be identified in a rapid and multiplexed fashion. In the present study, protein microarraying was combined with photoresist lithography to enable printing of extracellular matrix (ECM) protein arrays while precisely controlling "on-the-spot" cell-cell interactions. In this surface engineering approach, the micropatterned photoresist layer formed on a glass substrate served as a temporary stencil during the microarray printing, defining the micrometer-scale dimensions and the geometry of the cell-adhesion domains within the printed protein spots. After removal of the photoresist, the glass substrates contained micrometer-scale cell-adhesive regions that were encoded within 300 or 500 microm diameter protein domains. Fluorescence microscopy and atomic force microscopy (AFM) were employed to characterize protein micropatterns. When incubated with micropatterned surfaces, hepatic (HepG2) cells attached on 300 or 500 mum diameter protein spots; however, the extent of cell-cell contacts within each spot varied in accordance with dimensions of the photoresist stencil, from single cell...Continue Reading

References

Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A Ben-Ze'evS R Farmer
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T NakamuraA Ichihara
Nov 7, 1982·Journal of Theoretical Biology·M J BissellG Parry
Feb 1, 1994·The Journal of Cell Biology·S M Frisch, H Francis
Apr 29, 1994·Science·R SinghviD E Ingber
Oct 1, 1995·Current Opinion in Cell Biology·M Takeichi
Nov 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·F BerthiaumeM L Yarmush
Feb 1, 1997·Journal of Biomedical Materials Research·S N BhatiaM Toner
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·S A LelièvreM J Bissell
Jan 26, 1999·Annals of the New York Academy of Sciences·Z Werb, J R Chin
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·S TakayamaG M Whitesides
Nov 2, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S N BhatiaM Toner
Aug 22, 2000·Journal of Biomedical Materials Research·A FolchM Toner
Jul 12, 2001·Annual Review of Biomedical Engineering·G M WhitesidesD E Ingber
Sep 15, 2001·Journal of Biomaterials Science. Polymer Edition·N KrastevaG Altankov
Nov 10, 2001·Annual Review of Biomedical Engineering·A Folch, M Toner
Mar 2, 2002·Science·Maria SchönbächlerDetlef Günther
Nov 27, 2002·Langmuir : the ACS Journal of Surfaces and Colloids·A RevzinM V Pishko
Feb 6, 2004·Journal of Colloid and Interface Science·Elzbieta PamułaPaul G Rouxhet
Jun 3, 2004·Proteomics·Michael LynchEric Henderson
Jun 15, 2004·Nature Biotechnology·Daniel G AndersonRobert Langer
Jul 2, 2004·Nature Biotechnology·Sheng Ding, Peter G Schultz
Mar 23, 2005·Nature Methods·Christopher J FlaimSangeeta N Bhatia
May 7, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Alexander RevzinMehmet Toner
Feb 7, 2006·Biomaterials·Didier FalconnetMarcus Textor
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ali KhademhosseiniJoseph P Vacanti
Jul 28, 2006·Nature·Jamil El-AliKlavs F Jensen

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Citations

Mar 21, 2009·Journal of Materials Science. Materials in Medicine·Santiago A Rodríguez-SeguíJosep Samitier
Apr 2, 2010·ACS Applied Materials & Interfaces·Sunny S ShahAlexander Revzin
Mar 12, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Ji Youn LeeAlexander Revzin
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Ji Youn LeeAlexander Revzin
Sep 8, 2010·Lab on a Chip·Thomas BraschlerPhilippe Renaud
May 15, 2013·Expert Opinion on Drug Delivery·Saradha ChandrasekharVasant V Ranade
Dec 29, 2015·Trends in Biotechnology·Chun-Yen SungChao-Min Cheng
Dec 17, 2009·Colloids and Surfaces. B, Biointerfaces·Jinbo GanZhongkui Wu
May 30, 2009·Biomaterials·Qian ChengKyriakos Komvopoulos
Aug 30, 2008·Current Opinion in Biotechnology·Shara M DellatoreWilliam M Miller
Aug 5, 2008·Biosensors & Bioelectronics·Kevin A HeyriesChristophe A Marquette
Mar 17, 2009·Biochemical and Biophysical Research Communications·Gulnaz StybayevaAlexander Revzin
Jun 17, 2015·Advanced Materials·Hu TaoFiorenzo G Omenetto
Jan 22, 2010·Lab on a Chip·Scott A Zawko, Christine E Schmidt
Sep 13, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Mu-Gi WuHsien-Yeh Chen
Jul 31, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·C A CustódioA del Campo
Oct 9, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Chang-Ying XueKun-Lin Yang
Nov 3, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Zhenling ChenXingyu Jiang
Jun 9, 2018·ACS Applied Materials & Interfaces·Justyna BorowiecAndreas Schober
Mar 15, 2011·ACS Combinatorial Science·Albert R LiberskiUlrich S Schubert
Oct 24, 2018·The Journal of Physical Chemistry. B·Jiali ZhangGang-Yu Liu
Nov 18, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Sajan D GeorgeDeepak Mathur

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