Use of phylogenetic analysis of hepatitis C virus (HCV) hypervariable region 1 sequences to trace an outbreak of HCV in an autodialysis unit.

Journal of Clinical Microbiology
Philippe HalfonYvon Berland

Abstract

Hemodialysis patients are at high risk of infection by hepatitis C virus (HCV). The aim of this study was to investigate an HCV outbreak that occurred in an autodialysis unit by using epidemiological and molecular methods. Seroconversion to HCV antibody (anti-HCV) was observed in two patients over an 18-month period; two other patients had previously been recorded as anti-HCV positive. All four patients involved in the outbreak were tested for HCV RNA, and hepatitis C genotype determination was accomplished by a reverse hybridization assay. Furthermore, part of hypervariable region 1 (HVR1) of the hepatitis C genome was amplified and sequenced in samples from all HCV RNA-positive patients. Phylogenetic analysis of the nucleotide sequences obtained was carried out in order to investigate any possible epidemiological linkages among patients. The nucleotide sequences of the HVR1 regions of both newly infected patients were found to be identical to sequences of samples from previously recorded anti-HCV-positive original patients, suggesting that they were infected by the same isolate. Molecular and epidemiological analysis suggested that nosocomial patient-to-patient transmission was the most likely explanation for the virus spread...Continue Reading

References

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Citations

Jul 30, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Emmanuelle GirouJean-Michel Pawlotsky
Feb 5, 2013·Viral Immunology·Fatma Houissa KchoukTaieb Ben Abdallah
Jun 16, 2005·Journal of Gastroenterology and Hepatology·Takashi IshikawaTatsuo Miyamura
Jul 15, 2003·Clinical Microbiology Reviews·David K Henderson
Oct 30, 2015·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·L S Aho-GléléC Manoha
Feb 6, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·David KiangDavid P Schnurr
Sep 29, 2007·Alimentary Pharmacology & Therapeutics·A AbergelC Henquell
Aug 21, 2008·Journal of Medical Virology·Donatella FerraroRosa Di Stefano
Oct 8, 2005·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Annunziata FaustiniCarlo A Perucci
Jul 21, 2005·Journal of Medical Virology·Jacques IzopetLionel Rostaing
Oct 10, 2015·The International Journal of Artificial Organs·Fabrizio Fabrizi, Piergiorgio Messa
Dec 31, 2008·The International Journal of Artificial Organs·F FabriziP Martin
Sep 25, 2015·World Journal of Gastroenterology : WJG·Leila Ksiaa CheikhrouhouTaieb Ben Abdallah
Feb 3, 2005·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Vana SypsaAngelos Hatzakis

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