PMID: 8953676Jan 1, 1996Paper

Use of polymerase chain reaction for primary diagnosis of pulmonary tuberculosis in the clinical laboratory

Scandinavian Journal of Infectious Diseases
A R BahrmandM H Babaei

Abstract

A nested polymerase chain reaction (PCR) has been used for the rapid detection of tubercule bacilli in respiratory specimens from 287 patients suspected of tuberculosis. The results of PCR testing were compared with isolation methods (conventional culture and Bactec system) in 110 smear-positive and 177 smear-negative patients. There were only 4 false negative results by PCR in the 171 specimens that were M. tuberculosis complex culture-positive. Of 92 PCR-positive samples prepared from the smear-positive specimens 90 (97.8%) were confirmed by culture. However, a poor correlation was obtained between initial 122 PCR-positive results and combined 81 culture recovered organisms in smear-negative patients. After verification of the efficacy of isolation method, retesting PCR-positive culture-negative samples, and studies of patients' clinical histories, only 18 of the cases were found to be associated with the disease. The other 29 results out of the original 47 discrepants were considered PCR false positives, possibly due to contamination. In conclusion, the PCR assay described is suitable for implementation in daily routine work with respiratory specimens, however it should be validated with culture, especially for the smear-neg...Continue Reading

References

Feb 1, 1992·Acta Paediatrica·M NaritaH Kobayashi
Oct 11, 1991·Molecular and Cellular Probes·V Sritharan, R H Barker
Jun 1, 1990·Journal of Clinical Microbiology·P W HermansJ D van Embden
Feb 1, 1994·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·R R CutlerP Wilson
Apr 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·E Wolinsky
Jan 1, 1996·Scandinavian Journal of Infectious Diseases·A R BahrmandM H babaei
Dec 1, 1960·The American Journal of Medicine·L C CHRISTIANSON, H J DEWLETT

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Citations

Nov 25, 2005·International Urology and Nephrology·Vikram KalraSuresh Chand Dash
Nov 21, 2001·Journal of Microbiological Methods·S MitaraiH Shishido
Jul 5, 2003·Journal of Clinical Microbiology·Olga L SarmientoWilliam C Miller

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