PMID: 9191542Jun 1, 1997

Use of polymerase chain reaction to identify pneumococcal infection associated with hemorrhage and shock in two previously healthy young children

Clinical Chemistry
T O MessmerB S Fields

Abstract

A PCR assay was developed for detection of Streptococcus pneumoniae in clinical specimens including blood and paraffinized tissues. We were able to detect one organism of purified DNA or 4.5 colony-forming units in blood. The primers did not cross-react with other upper respiratory tract streptococci or with pathogens commonly found in clinical specimens. This assay was used in an investigation of an outbreak of severe illness characterized by septic shock and hemorrhage in previously healthy children. PCR detected S. pneumoniae in cerebrospinal fluid and autopsy tissues of the two infants who died. The findings from this assay indicated that PCR offers increased specificity and sensitivity over latex agglutination and counterimmunoelectrophoresis and should prove useful in the identification of additional cases of severe illness caused by S. pneumoniae.

Related Concepts

Metazoa
DNA, Bacterial
Hemorrhage
Pneumococcal Infections
Statistical Sensitivity
Dall Sheep
Endotoxic Shock
Streptococcus pneumoniae
Nested Polymerase Chain Reaction
In Vitro [Publication Type]

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