Use of polyurethane minisponges to collect human tear fluid

Cornea
Juan López-CisternasRemigio O López-Solís

Abstract

To characterize a method of tear collection based on the use of amphiphilic polyurethane absorbing minisponges. Tear fluid was collected from 17 healthy volunteers. A preweighed polyurethane dry minisponge was laid on the margin of the lower eyelid. Once wet (5-10 minutes), the fluid was transferred to a preweighed Eppendorf tube after squeezing the sponge by centrifugation. The amount of fluid absorbed and fluid recovered were determined by reweighing the sponge and the tube after absorption and centrifugation steps, respectively. The fluid was qualitatively characterized by electrophoretic polypeptide profiling in Coomassie blue-stained SDS-polyacrylamide gels. Per eye, 14.6 +/- 5.3 microL tear fluid was collected. That volume was about 90% of the fluid absorbed by polyurethane minisponges, almost doubling the fraction recovered from other more hydrophilic absorbing polymers. Major bands characterizing the electrophoretic profile of this fluid were those of 79, 66, 27, 18, and 14 kd. This profile was indistinguishable from that of tear fluid aspirated into glass microcapillaries. Tear fluid collected simultaneously from both eyes displayed the same profiles. Successive tear samples from a single eye showed the same profile ex...Continue Reading

References

Oct 1, 1969·Analytical Biochemistry·S BramhallJ R Loewenberg
Jul 1, 1966·American Journal of Ophthalmology·L T Jones
Jul 1, 1983·American Journal of Ophthalmology·A Berta
Sep 1, 1981·Survey of Ophthalmology·N J Van Haeringen
Sep 1, 1980·Ophthalmology·A Jordan, J Baum
Nov 1, 1980·American Journal of Ophthalmology·M R AllansmithM B Abelson
Apr 1, 1997·Acta Ophthalmologica Scandinavica·T VirtanenT Tervo
Aug 1, 1997·Current Eye Research·M SakataA R Beaton
Jul 10, 1999·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·J W OhH B Lee
Jan 10, 2001·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·V NgA Lee
May 9, 2002·Cornea·Hitoshi Watanabe
Aug 31, 2004·Journal of Materials Science. Materials in Medicine·P V JacksonP Gilson
Oct 13, 2004·Journal of Colloid and Interface Science·Matthew D MontminyChristopher W Macosko

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Citations

Jun 2, 2007·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Flavio MantelliStefano Bonini
Feb 4, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Kaijun LiKaili Wu
Dec 17, 2008·Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)·Marieh EsmaeelpourPaul J Murphy
Jul 17, 2014·Biological Research·Leonidas Traipe-CastroRemigio López-Solís
Jun 27, 2012·Progress in Retinal and Eye Research·Lei Zhou, Roger W Beuerman
Aug 3, 2013·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Aaron BarrettBarbara C Trask
May 27, 2016·Analytical and Bioanalytical Chemistry·Vitaly AvilovScott A Shippy
Jun 4, 2016·Biological Research·Felipe Traipe-SalasRemigio López Solís
Feb 26, 2015·Anais Da Academia Brasileira De Ciências·Juliana L DreyfussJoão R Martins
Apr 8, 2017·Allergy·A LeonardiV Calder
Aug 29, 2017·Veterinary Ophthalmology·Lionel SebbagJonathan P Mochel
Nov 15, 2017·Current Opinion in Allergy and Clinical Immunology·Sveva CastelliPaolo M Matricardi
Jan 1, 2014·Eye and Vision·Sharon D'Souza, Louis Tong
Jan 7, 2017·Cornea·Felipe TraipeRemigio López-Solís
May 1, 2018·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Lionel SebbagJonathan P Mochel
Apr 1, 2020·Lab on a Chip·Zachary BallardAydogan Ozcan
Feb 27, 2021·European Journal of Ophthalmology·Janusz PieczyńskiJolanta Kiewisz
Feb 18, 2018·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Roshni PatelDanielle M Robertson
Nov 10, 2021·Acta Ophthalmologica·Janika NättinenHannu Uusitalo

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