Use of porcine vaginal tissue ex-vivo to model environmental effects on vaginal mucosa to toxic shock syndrome toxin-1

Toxicology and Applied Pharmacology
Catherine C DavisChristopher A Squier

Abstract

Menstrual toxic shock syndrome (mTSS) is a rare, recognizable, and treatable disease that has been associated with tampon use epidemiologically. It involves a confluence of microbial risk factors (Staphylococcus aureus strains that produce the superantigen-TSST-1), as well as environmental characteristics of the vaginal ecosystem during menstruation and host susceptibility factors. This paper describes a series of experiments using the well-characterized model of porcine vaginal mucosa ex-vivo to assess the effect of these factors associated with tampon use on the permeability of the mucosa. The flux of radiolabeled TSST-1 and tritiated water ((3)H2O) through porcine vaginal mucosa was determined at various temperatures, after mechanical disruption of the epithelial surface by tape stripping, after treatment with surfactants or other compounds, and in the presence of microbial virulence factors. Elevated temperatures (42, 47 and 52°C) did not significantly increase flux of (3)H2O. Stripping of the epithelial layers significantly increased the flux of labeled toxin in a dose-dependent manner. Addition of benzalkonium chloride (0.1 and 0.5%) and glycerol (4%) significantly increased the flux of (3)H2O but sodium lauryl sulfate at...Continue Reading

References

May 1, 1992·Pharmaceutical Research·R O Potts, R H Guy
Jun 1, 1982·Annals of Internal Medicine·G Wagner, B Ottesen
Feb 1, 1983·The Journal of Infectious Diseases·P M Schlievert, D A Blomster
Apr 1, 1981·The Journal of Infectious Diseases·P M SchlievertR D Nishimura
Jun 1, 1982·Annals of Internal Medicine·C C LinnemannA Beiting
Dec 18, 1980·The New England Journal of Medicine·J P DavisM LaVenture
Jul 1, 1995·Clinical Microbiology Reviews·M Kotb
May 7, 1998·Chemistry and Physics of Lipids·P W Wertz, B van den Bergh
Jan 11, 2000·Clinical Microbiology Reviews·M M DingesP M Schlievert
Mar 14, 2000·The Journal of Pharmacy and Pharmacology·D DimitrijevicA T Florence
Mar 16, 2002·Infection and Immunity·Jeffrey W ShuppCarol H Pontzer
Apr 15, 2003·Journal of Endotoxin Research·Alexander H Dalpke, Klaus Heeg
Nov 7, 2003·Journal of Pharmaceutical Sciences·Joseph A NicolazzoBarrie C Finnin
Jan 6, 2004·Journal of Pharmaceutical Sciences·Joseph A NicolazzoBarrie C Finnin
Jun 4, 2005·Journal of Applied Physiology·Donna R HillThomas W Osborn
Nov 25, 2005·Current Drug Delivery·Pieter van der Bijl, Armorel D van Eyk
Nov 29, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Seyoum AyehunieMitchell Klausner
Aug 28, 2007·Journal of Pharmaceutical Sciences·Christopher A SquierCatherine C Davis
Nov 21, 2007·Biochemistry·Patrick M SchlievertBruce E Jones
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Pawel Mak
Jun 17, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Darrell R BoverhofMichael R Woolhiser
Oct 14, 2008·Anaerobe·Alla AroutchevaSebastian Faro
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amanda J BrosnahanPatrick M Schlievert
Jun 17, 2009·American Journal of Reproductive Immunology : AJRI·Li YuShangwu Chen
Mar 26, 2010·Clinical and Vaccine Immunology : CVI·Patrick M SchlievertBruce E Jones
Nov 23, 2010·BMC Infectious Diseases·Thomas R MoenchRichard A Cone
May 4, 2011·The FEBS Journal·Amanda J Brosnahan, Patrick M Schlievert
Sep 29, 2011·Alternatives to Laboratory Animals : ATLA·Gertrude-Emilia CostinRoger D Curren
Aug 25, 2012·Frontiers in Cellular and Infection Microbiology·Michele J AndersonMarnie L Peterson

❮ Previous
Next ❯

Citations

Feb 18, 2015·Advanced Drug Delivery Reviews·Rita Monteiro MachadoRita Palmeira-de-Oliveira
May 31, 2018·Pathogens·Stephen W TuffsJohn K McCormick
May 29, 2020·Clinical Microbiology Reviews·Patrick M Schlievert, Catherine C Davis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Epidemiologie, mikrobiologie, imunologie : casopis Spolecnosti pro epidemiologii a mikrobiologii Ceské lékarské spolecnosti J.E. Purkyne
P PetrásP Prásil
Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete
W C MarschF J Fehrenbach
© 2021 Meta ULC. All rights reserved