Use of porous alginate sponges for substantial chondrocyte expansion and matrix production: effects of seeding density

Biotechnology Progress
Chi-Nan YenYu-Chen Hu

Abstract

Autologous cell transplantation is a promising approach for cartilage repair, but the expansion of chondrocytes in a monolayer, a common approach to amplifying the cell number, inevitably leads to cell de-differentiation. To explore whether porous alginate sponges could be utilized for chondrocyte expansion and investigate the effects of seeding densities, the porcine chondrocytes were seeded to porous alginate sponges at low (5 x 10(5) cells per 40 sponges), medium (5 x 10(6) cells per 40 sponges), or high (2 x 10(7) cells per 40 sponges) density. After 4-week perfusion culture, all three groups resulted in chondrocyte proliferation, maintenance of chondrocytic gene (collagen II, Sox 9 and aggrecan) expression, and formation of cell clusters resembling cartilaginous tissues. The higher the seeding density, the higher the final cell density and GAGs production and, accordingly, the larger the cell clusters. Strikingly, the cumulative expansion ratios achieved by the low-density group ( approximately 150-fold) significantly exceeded those achieved by the medium (approximately 21-fold) and high (approximately 4.7-fold) density groups, as well as those achieved using other scaffolds. In conclusion, seeding chondrocytes to the algi...Continue Reading

Citations

Nov 7, 2008·Journal of Materials Science. Materials in Medicine·R Seda Tiğli, Menemşe Gümüşderelioğlu
Oct 16, 2013·Tissue Engineering. Part a·Therese AndersenBjørn E Christensen
Mar 19, 2009·Biotechnology Progress·Aasma A KhanStephen D Waldman
May 28, 2010·Arthritis and Rheumatism·Martin K LotzDarryl D'Lima
Feb 19, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yoshiaki HoshiyamaMasashi Neo
Dec 17, 2014·Journal of Biomaterials Science. Polymer Edition·Jeong Eun SongGilson Khang

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