Use of procaine and procainamide as derivatizing co-matrices for the analysis of oligosaccharides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Hélène Lavanant, Corinne Loutelier-Bourhis

Abstract

Analysis of oligosaccharides by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry often yields only alkali metal cation adducts, which results in lower fragmentation yields and difficulty to retrieve sequence information. Derivatization by reductive amination may be used to promote Y-type glycosidic cleavages. However, this involves time-consuming preparations and purifications with sample loss. Here, procaine and procainamide were used directly as co-matrices with 2,5-dihydroxybenzoic acid (DHB). Acidified 10 g/L procaine hydrochloride or procainamide hydrochloride solutions in water/acetonitrile were added to the oligosaccharide solution one minute before preparing our MALDI targets using DHB with the dried-droplet method. This simple protocol resulted in deposits of very fine homogeneous crystals. Positive ion mass spectra, easily acquired in an automated mode, presented a high percentage of oligosaccharides derivatized as Schiff base or glycosylamine notably detected as protonated molecules [M + H](+). The high abundance of procaine or procainamide on the target did not impede the ionization process, improved the signal-to-noise ratio and eliminated the need to search for 'sweet spots'. Fragmentation of ...Continue Reading

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Citations

Mar 28, 2013·Chemical Reviews·William R AlleyMilos V Novotny
Oct 16, 2015·Rapid Communications in Mass Spectrometry : RCM·Hang YuanYufen Zhao
Oct 24, 2018·Canadian Review of Sociology = Revue Canadienne De Sociologie·Johanne Jean-Pierre
Feb 10, 2021·Critical Reviews in Analytical Chemistry·Vladimir G Zaikin, Roman S Borisov
Jun 22, 2019·Carbohydrate Polymers·Phu Cuong NguyenJong-Tae Park

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