Use of processing fluid samples for longitudinal monitoring of PRRS virus in herds undergoing virus elimination

Porcine Health Management
Giovani TrevisanDaniel C L Linhares

Abstract

This was an observational study that prospectively followed 29 breeding herds for 65 weeks in the U.S.A. that became infected with porcine reproductive and respiratory syndrome virus (PRRSv). The herds operated in a four-week batch farrowing system and adopted a load-close-expose strategy using a modified-live virus vaccine to achieve PRRSv stability. The purpose of this study was to describe time to stability (TTS) based on RT-qPCR testing for PRRSv RNA on processing fluid samples in herds undergoing PRRSv elimination, after implementing herd closure and mass exposure to a PRRS modified-live virus (MLV) vaccine. For the purpose of this study, stability was defined as consistently producing PRRSv-negative pigs. Study herds were monitored until two consecutive piglet batches tested PRRSv RT-qPCR negative, then 30 due-to-wean piglet sera from the second batch were tested for PRRSv RNA by RT-qPCR. Once the farm re-opened, sera from incoming naïve gilts were tested for anti-PRRSv antibodies by ELISA at 30- and 60-days post-entry to confirm negative status to PRRSv. Day zero was the day of whole-herd exposure to a commercial PRRS vaccine virus. Twenty-eight of 29 herds (96.55%) achieved TTS within the study period. TTS ranged from 1...Continue Reading

References

Feb 1, 2018·Eating Disorders·Carolyn M PearsonCarol B Peterson

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Citations

Jul 21, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Hanna Turlewicz-PodbielskaMałgorzata Pomorska-Mól
Jul 15, 2020·Animals : an Open Access Journal From MDPI·Ilaria Di BartoloFabio Ostanello
Jun 29, 2021·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Giovani TrevisanDaniel C L Linhares
Nov 9, 2021·Transboundary and Emerging Diseases·Magnus R CamplerAndréia G Arruda

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Methods Mentioned

BETA
ELISA
PCR
castration

Software Mentioned

SAS

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