PMID: 9523849Apr 2, 1998Paper

Use of proliferating cell nuclear antigen as a marker of liver regeneration after partial hepatectomy in rats

The Journal of Laboratory and Clinical Medicine
N AssyG Y Minuk

Abstract

In order to document and compare proliferating cell nuclear antigen (PCNA) mRNA and protein levels with more traditional parameters of hepatic regenerative activity in a rat model, adult male Sprague-Dawley rats (4 to 6 per group) were killed at various times up to 96 hours after 70% partial hepatectomy. At each time interval, tissue PCNA mRNA abundance and protein levels were documented (by Northern and Western blot analysis, respectively) and compared with the results of PCNA immunostaining and 3H-thymidine incorporation into hepatic DNA. Tissue PCNA protein levels were also documented in additional groups of rats 12, 24, 36, and 48 hours after sham or 30% partial hepatectomy. PCNA mRNA expression after partial hepatectomy was variable: almost undetectable at 24 hours, levels returned to baseline at 36 hours, then fell again to low levels at 96 hours. PCNA protein levels remained stable for 36 hours, increased to fourfold above baseline (p < 0.01) at 48 hours, then remained elevated for the duration of the 96-hour study. Changes in PCNA by immunostaining were similar but tended to occur somewhat earlier (significant increases being detectable at 24 hours), whereas 3H-thymidine incorporation detected the earliest increases in ...Continue Reading

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Citations

Apr 28, 2011·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Quan-hui YangBo Tang
Aug 12, 2006·Digestive Diseases and Sciences·Manna ZhangGerald Y Minuk
Nov 30, 2011·The Journal of Physiological Sciences : JPS·San-Qiang LiXue-Liang Chen
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Apr 1, 1997·Journal of Hepatology·N Assy, G Y Minuk
Jul 27, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ian R CorbinGerald Y Minuk
May 3, 2006·Biological & Pharmaceutical Bulletin·Masaki KobayashiKeijiro Samejima
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Jul 1, 2008·Gastroenterology·Diarmaid Dominic Houlihan, Philip Noel Newsome
Jan 29, 2008·The Journal of Surgical Research·Ian P J AlwaynMark Puder
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