Use of pulsed-field gel electrophoresis to investigate an outbreak of Serratia marcescens.

Journal of Clinical Microbiology
Z Y ShiB S Hu

Abstract

Pulsed-field gel electrophoresis (PFGE) typing was applied to the epidemiological investigation of 20 Serratia marcescens isolates collected from urine specimens of 17 patients and three urinals over a 2-month period. Twenty-five epidemiologically unrelated strains were also tested to determine the discriminatory power of PFGE. The PFGE fingerprints of each isolate were consistent in three different tests. The 20 outbreak isolates had an identical PFGE fingerprint pattern, while the epidemiologically unrelated strains demonstrated unique PFGE fingerprint patterns. The source of the outbreak was inadequately disinfected urinals. We conclude that PFGE served as a highly discriminatory and reproducible method for the epidemiological investigation of the outbreak of S. marcescens infection addressed by this study.

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Citations

Jul 8, 1999·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·H Peltroche-LlacsahuangaG Haase
Aug 5, 2000·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·G DorseyD R Bangsberg
Jan 5, 2002·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·P VillariA Scarcella
Jul 19, 2006·Clinical Microbiology Reviews·Aparajita SinghMarcus J Zervos
Oct 7, 2011·Clinical Microbiology Reviews·Steven D Mahlen
Jul 10, 2012·The Pediatric Infectious Disease Journal·Jennifer VillaFernando Chaves
Aug 16, 2019·Antimicrobial Resistance and Infection Control·Laura MolesJuan M Rodríguez
Dec 1, 1998·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·F LuzzaroL Pagani
Nov 3, 2006·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Jutte J C de VriesJan P Arends
Aug 23, 2011·PloS One·Kathryn Patterson SutherlandErin K Lipp
Oct 9, 2003·Journal of Clinical Microbiology·Lin-Hui SuUNKNOWN Infection Control Group

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