Use of pulsed field gel electrophoresis to determine the source of microbial contamination of central venous catheters

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
M A LivesleyT S Elliott

Abstract

Microorganisms detected in situ on the distal tip of central venous catheters (CVC) within 90 min of insertion were investigated using pulsed-field gel electrophoresis to analyse genomic fragments obtained with the SmaI restriction enzyme. Thirty patients received a triple lumen CVC, which was inserted directly through the skin using the Seldinger technique. In a further 30 patients a triple lumen CVC was inserted through a Swan sheath, thereby avoiding direct contact of the CVC with the skin. Staphylococci were isolated from the distal tips of the catheters in 6 patients (5 who had the CVC inserted directly through the skin and 1 who had the CVC inserted via a Swan sheath.) Twenty-three staphylococcal isolates were also isolated from the insertion equipment and the skin swabs surrounding the insertion site of these six patients. All the isolates were genotyped. In one of the patients the organisms isolated from the skin were identical to those on the CVC tip. In two further patients similar organisms were isolated from the insertion equipment and the patients' skin. These results, in addition to the reduced colonisation rates observed when catheters were introduced through a Swan sheath, support the hypothesis that microorgani...Continue Reading

References

Apr 15, 1992·FEMS Microbiology Letters·B LinaJ Fleurette
Nov 1, 1988·Journal of Medical Microbiology·T S Elliott
Mar 1, 1995·Journal of Medical Microbiology·B LinaJ Fleurette
Nov 1, 1993·The Journal of Infection·W K LiuT S Elliott
Feb 1, 1993·The Journal of Antimicrobial Chemotherapy·S E Tebbs, T S Elliott
Mar 1, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·T S ElliottM H Faroqui

❮ Previous
Next ❯

Citations

Aug 18, 1999·Journal of Clinical Pathology·B M DobbinsM H Wilcox
May 4, 2004·The Lancet Infectious Diseases·Silvia F CostaElias J Anaissie
Aug 23, 2005·The Journal of Infection·Tony Worthington, Tom S J Elliott
Mar 5, 2013·Neurological Research·Federico BilottaGiovanni Rosa
Dec 3, 1999·The Journal of Hospital Infection·S LangT S Elliott
Jan 3, 2006·JPEN. Journal of Parenteral and Enteral Nutrition·Marcia Ryder
Nov 27, 1998·The Journal of Hospital Infection·T S Elliott, S E Tebbs
Sep 23, 2003·Anesthesia and Analgesia·Richard C Prielipp, Robert J Sherertz
Sep 23, 2003·Anesthesia and Analgesia·Christian JeskeArnulf Benzer
Apr 1, 2010·Journal of Indian Society of Periodontology·D Gandhimadhi, R Mythili
Dec 8, 2009·British Journal of Hospital Medicine·C T CrosbyD Adams

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
T S ElliottM H Faroqui
Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America
A VossJ F Meis
American Journal of Diseases of Children
D B Nelson, J S Garland
European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
B LinaJ Etienne
© 2022 Meta ULC. All rights reserved