Use of pulsed gradient spin-echo NMR as a tool in MALDI method development for polymer molecular weight determination

Analytical Chemistry
Michaël MazarinLaurence Charles

Abstract

This study shows how mass spectrometry and pulsed gradient spin-echo (PGSE) nuclear magnetic resonance can be advantageously combined to achieve more reliable molecular weight information for polymers. Specifically, PGSE was shown to be a convenient tool for a rapid evaluation of Mw values to be further used as guidelines in matrix-assisted laser desorption/ionization (MALDI) sample preparation. PGSE calibration curves, established under given experimental conditions, were shown to be particularly robust, as they could be applied satisfactorily on different commonly available NMR instruments and different time frames. PGSE results were shown to compare well with size exclusion chromatography data used as a reference to validate this alternative technique. Moreover, because PGSE is relatively fast, it can be used interactively with MALDI analysis to check and understand mass spectrum profiles. This approach was first tested on poly(methyl methacrylate) (PMMA) standards and then successfully applied to determine the molecular weight of two unknown samples, a PMMA and a poly(ethylene glycol) monomethacrylate polymer.

References

Apr 14, 2000·Analytical Chemistry·R SkeltonR Zenobi
Jul 11, 2001·Journal of Magnetic Resonance·J P StampsR Hulst
Jul 6, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Alexander BootzJörg Kreuter

❮ Previous
Next ❯

Citations

Dec 17, 2008·Pharmaceutical Research·Esmaiel Jabbari
Oct 24, 2009·Analytica Chimica Acta·Stéphane VielDenis Bertin
Mar 6, 2010·Journal of the American Society for Mass Spectrometry·Rémi GiordanengoLaurence Charles
Nov 17, 2007·Journal of Magnetic Resonance·Stéphane VielStefano Caldarelli
Jan 29, 2017·Journal of Colloid and Interface Science·Nathan H WilliamsonMagnus Nydén
Jan 3, 2007·Journal of Mass Spectrometry : JMS
Apr 14, 2007·Magnetic Resonance in Chemistry : MRC·Christopher A Crutchfield, Douglas J Harris
Jan 31, 2018·Nanoscale·Yanling ZhaiShaojun Dong
May 20, 2008·Analytical Chemistry·Steffen M Weidner, Sarah Trimpin
Sep 12, 2009·Analytical Chemistry·Caroline BarrèreLaurence Charles

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.