Use of quantitative broad-based polymerase chain reaction for detection and identification of common bacterial pathogens in cerebrospinal fluid.

Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine
Richard RothmanCharlotte Gaydos

Abstract

Conventional laboratory diagnosis of bacterial meningitis based on microscopy followed by culture is time-consuming and has only moderate sensitivity. The objective was to define the limit of detection (LOD), analytic specificity, and performance characteristics of a broad-based quantitative multiprobe polymerase chain reaction (PCR) assay for rapid bacterial detection and simultaneous pathogen-specific identification in patients with suspected meningitis. A PCR algorithm consisting of initial broad-based detection of Eubacteriales by a universal probe, followed by pathogen identification using either pathogen-specific probes or Gram-typing probes, was employed to detect pathogens. The 16S rRNA gene, which contains both conserved and variable regions, was chosen as the target. Pathogen-specific probes were designed for Streptococcus pneumoniae, Neisseria meningitidis, Haemophilus influenzae, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, and Listeria monocytogenes. Gram-positive and -negative typing probes were designed based on conserved regions across all eubacteria. The LOD and time to detection were assessed by dilutional mocked-up samples. A total of 108 convenience cerebrospinal fluid (CSF) clinical ...Continue Reading

References

Oct 1, 1978·Journal of Clinical Pathology·B Chattopadhyay, E Thomas
May 1, 1997·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·D J Weber, W A Rutala
Jun 10, 1998·Journal of Clinical Microbiology·S A DunbarJ E Clarridge
Jul 17, 1998·Journal of Clinical Microbiology·P KotilainenS Nikkari
Jul 25, 2000·Journal of Clinical Laboratory Analysis·J H AtobeE M Mamizuka
Jan 25, 2002·Archives of Disease in Childhood·S J HackettC A Hart
Dec 20, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Louis D SaravolatzBradley Belian
Jun 24, 2003·Archives of Disease in Childhood·H El BashirR Booy
Nov 25, 2003·The Journal of Hospital Infection·A AlothmanE Williams
Jun 3, 2004·The Lancet Infectious Diseases·Samuel Yang, Richard E Rothman
Oct 29, 2004·The New England Journal of Medicine·Morton N Swartz
Dec 23, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·John B RobbinsEmil C Gotschlich
Jul 8, 2005·Journal of Clinical Microbiology·Sven PoppertNele Wellinghausen
Sep 30, 2006·Research in Microbiology·Dhyanesh DesaiAsha Shenoy
May 4, 2007·Indian Journal of Pediatrics·Pratibha SinghiSunit Singhi
Jul 5, 2007·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E Van GastelA Mertens
Feb 29, 2008·Journal of Clinical Microbiology·Samuel YangRichard E Rothman
Mar 5, 2008·The Pediatric Infectious Disease Journal·Mark I NeumanMarvin B Harper
Sep 16, 2008·The Journal of Emergency Medicine·Wasiulla RafiSursarla Krishna Shankar

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Citations

Apr 6, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Jeannette GuarnerSherif R Zaki
Sep 16, 2011·Journal of Clinical Microbiology·Alban Le MonnierSamer Kayal
Jan 25, 2013·Journal of Clinical Microbiology·Milena EulerManfred Weidmann
Feb 13, 2016·Diagnostic Microbiology and Infectious Disease·Adriana CalderaroFlora De Conto
Aug 28, 2012·Annals of Emergency Medicine·Richard E RothmanCharlotte A Gaydos
Jan 31, 2012·Neurologic Clinics·Amy A Pruitt
Nov 19, 2011·Transplant Infectious Disease : an Official Journal of the Transplantation Society·R BharadwajP H Chandrasekar
Jul 12, 2011·Biotechnology Advances·Seungkyung ParkSamuel Yang
Apr 3, 2019·Annual Review of Analytical Chemistry·Dong Jin ShinTza-Huei Wang
Aug 19, 2021·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Madiha FidaRobin Patel

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