Use of real-time quantitative polymerase chain reaction to monitor antibiotic therapy in a dog with naturally acquired Mycoplasma haemocanis infection

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
K Lisa Hulme-MoirSéverine Tasker

Abstract

Mycoplasma haemocanis is a hemotropic bacterium that can be associated with acute hemolytic disease in immunocompromised or splenectomized dogs. The present case report describes for the first time the use of real-time quantitative polymerase chain reaction (qPCR) to monitor M. haemocanis infection in a splenectomized dog. The report also describes the application of real-time qPCR for the analysis of deoxyribonucleic acid extracted from stained blood films. The analysis of blood films from the time of initial presentation allowed a retrospective confirmation of M. haemocanis infection. The M. haemocanis copy numbers remained high throughout antibiotic treatment of this dog. A decline in copy numbers was only recorded after 11 months of therapy, when improvements in clinical and hematological indices were also noted. Clearance of infection was not achieved, and the dog remained positive for M. haemocanis at 3.5 months postcessation of antibiotic therapy. Cytological examination of blood films for the presence of organisms was insensitive for the detection of parasitemia.

References

Dec 1, 1978·The Journal of Small Animal Practice·D C North
Jun 22, 2001·Journal of the American Veterinary Medical Association·J J Brinson, J B Messick
Jul 18, 2002·Journal of the American Veterinary Medical Association·Kristy L DowersMichael R Lappin
Aug 9, 2003·Journal of Clinical Microbiology·S TaskerT J Gruffydd-Jones
Jan 7, 2004·Journal of Microbiological Methods·Séverine TaskerMichael R Lappin
Mar 30, 2004·Veterinary Clinical Pathology·Joanne B Messick
Nov 6, 2004·Journal of Clinical Microbiology·Martin J KennySéverine Tasker
Aug 1, 2006·Veterinary Microbiology·Séverine TaskerTimothy J Gruffydd-Jones
Nov 23, 2006·Annals of the New York Academy of Sciences·Hisashi InokumaPhilippe Brouqui
Mar 14, 2008·Journal of Clinical Microbiology·Iain R PetersSéverine Tasker
Nov 19, 2008·Infection and Immunity·K GroebelL E Hoelzle
Jan 6, 2009·American Journal of Veterinary Research·Kristy L DowersMichael R Lappin
May 22, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J-L MariéB Davoust
Aug 31, 1935·The Journal of Experimental Medicine·J B McNaughtV Scott

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Citations

Mar 1, 2012·Journal of Bacteriology·Naíla C do NascimentoJoanne B Messick
Oct 2, 2012·Veterinary Research·Naíla C do NascimentoJoanne B Messick
Apr 9, 2016·The Veterinary Journal·S H CannonR Chandrashekar
Jul 25, 2013·The Journal of Small Animal Practice·K CrawfordS M Warman
Oct 30, 2010·The Journal of Small Animal Practice·S M WarmanS Tasker

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Methods Mentioned

BETA
PCR
transmission electron microscopy

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