Use of recombinant chimeric antigens for the serodiagnosis of Mycoplasma pneumoniae infection.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
Francesca MontagnaniNicola Gargano

Abstract

In this paper, we have evaluated the diagnostic utility of three antigenic regions of the Mycoplasma pneumoniae P1, P30, and MPN456 gene products in order to replace the soluble, whole-cell bacterial extract in serological assays. Antigenic regions, being previously identified as B-cell epitopes, were used individually or assembled in a recombinant chimeric antigen by genetic engineering. Paired serum samples from 47 patients with M. pneumoniae infection and from 39 subjects with a clinical picture of atypical pneumonia but without a defined diagnosis of M. pneumoniae infection were included. Immunoglobulin G (IgG) antibodies against epitopes carried by recombinant antigens were measured by performing recombinant enzyme-linked immunosorbent assays (Rec-ELISAs). Rec-ELISA results were compared to those obtained by a commercial assay using the whole-cell Mycoplasma antigen. Our study demonstrates that all IgG Rec-ELISAs using recombinant antigens have better sensitivity with respect to the commercial assay. Furthermore, we show that the use of chimeric antigens improve the performance of the assays. The use of recombinant antigens is effective in distinguishing M. pneumoniae-infected patients from uninfected individuals and shows...Continue Reading

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Citations

Jan 6, 2012·Clinical and Vaccine Immunology : CVI·Jianfang DaiJin Si
Feb 10, 2016·Frontiers in Microbiology·Roger Dumke, Enno Jacobs
Mar 7, 2018·Journal of Clinical Laboratory Analysis·Victoria Ortiz de la TablaFélix Gutiérrez
May 26, 2017·Clinical Microbiology Reviews·Ken B WaitesT Prescott Atkinson
Apr 15, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elisa Beghetto, Nicola Gargano
Mar 19, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Guanhua XueJing Yuan
Aug 27, 2021·Biosensors·Paola Alejandra Fiorani CeledonFred Luciano Neves Santos

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