Use of recombinant E protein domain III-based enzyme-linked immunosorbent assays for differentiation of tick-borne encephalitis serocomplex flaviviruses from mosquito-borne flaviviruses

Journal of Clinical Microbiology
Michael R HolbrookAlan D T Barrett

Abstract

The serological diagnosis of infection by flaviviruses is complicated by the presence of flavivirus cross-reactive antibodies that produce false-positive results for flavivirus infections, especially in regions where more than one virus is endemic. Current diagnostic reagents for tick-borne flavivirus infection have been found to cross-react with yellow fever- or dengue virus-positive sera. This study utilized recombinant flavivirus E protein domain 3 (rE-D3) as a diagnostic reagent to differentiate between infection by mosquito- and tick-borne flaviviruses. This study found that the use of rE-D3 in an enzyme-linked immunosorbent assay (ELISA)-based format allowed the differentiation between serum specific for either mosquito- or tick-borne flaviviruses, but not among the members of the tick-borne encephalitis (TBE) serocomplex of flaviviruses. Sera derived against several TBE serocomplex rE-D3 were found to cross-react with heterologous rE-D3 within the TBE serocomplex, but not with those from mosquito-borne flaviviruses, in both Western blots and ELISAs. Mouse hyperimmune sera generated against TBE serocomplex viruses were also found to react specifically with TBE serocomplex rE-D3, but not with rE-D3 from mosquito-borne viru...Continue Reading

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Citations

Mar 12, 2009·Archives of Virology·Kentaro Yoshii, Michael R Holbrook
Mar 13, 2013·Protein Engineering, Design & Selection : PEDS·Nora ZidaneHugues Bedouelle
Apr 17, 2012·Virology Journal·Davide LelliPaolo Cordioli
May 3, 2011·Emerging Infectious Diseases·Benjamin J BriggsJohn Hay
Nov 15, 2013·International Journal of Environmental Research and Public Health·Cécile BeckSylvie Lecollinet
Apr 3, 2014·Vector Borne and Zoonotic Diseases·Lehlohonolo Mathengtheng, Felicity J Burt
Sep 8, 2010·Biologicals : Journal of the International Association of Biological Standardization·Juliana Helena ChávezLuiz Tadeu Moraes Figueiredo
May 5, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Diana LudolfsHerbert Schmitz
Jan 6, 2011·Microbiology and Immunology·Ayae Ikawa-YoshidaIkuo Takashima
Dec 18, 2013·Microbiology and Immunology·Nozyechi N ChidumayoHiroaki Kariwa
Mar 23, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D WangH Jiang
Apr 7, 2012·Biotechnology Advances·C A MartínezJ Rodríguez Talou
Feb 5, 2014·Diagnostic Microbiology and Infectious Disease·Nozyechi N ChidumayoHiroaki Kariwa
Nov 1, 2012·Antiviral Research·Michael R Holbrook
Dec 2, 2014·Scientific Reports·Zhida LiuMingzhao Zhu
Feb 13, 2016·Clinical and Experimental Vaccine Research·Hossein FahimiMahshid Mohammadipour
Sep 1, 2006·Cell Biochemistry and Function·Agostino PuglieseDonato Torre
Jan 26, 2008·Journal of Agricultural and Food Chemistry·Youn-Young Shim, Janitha P D Wanasundara

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