Use of recombinant ESAT-6:CFP-10 fusion protein for differentiation of infections of cattle by Mycobacterium bovis and by M. avium subsp. avium and M. avium subsp. paratuberculosis

Clinical and Diagnostic Laboratory Immunology
W Ray WatersF C Minion

Abstract

Immunological diagnosis of Mycobacterium bovis infection of cattle is often confounded by cross-reactive responses resulting from exposure to other mycobacterial species, especially Mycobacterium avium. Early secretory antigenic target 6 (ESAT-6) and culture filtrate protein 10 (CFP-10) are dominant gamma interferon (IFN-gamma)-inducing antigens of tuberculous mycobacteria, and they are absent from many environmental nontuberculous mycobacteria. Because M. avium exposure is the primary confounding factor in the diagnosis of M. bovis-infected animals, in vitro responses to a recombinant ESAT-6:CFP-10 (rESAT-6:CFP-10) fusion protein by blood leukocytes from cattle naturally exposed to M. avium or experimentally challenged with Mycobacterium avium subsp. avium or Mycobacterium avium subsp. paratuberculosis were compared to responses by M. bovis-infected cattle. Responses to heterogeneous mycobacterial antigens (i.e., purified protein derivatives [PPDs] and whole-cell sonicates [WCSs]) were also evaluated. Tumor necrosis factor alpha (TNF-alpha), IFN-gamma, and nitric oxide responses by M. bovis-infected cattle to rESAT-6:CFP-10 exceeded (P < 0.05) the corresponding responses by cattle naturally sensitized to M. avium. Experimental...Continue Reading

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Citations

Jan 25, 2005·BMC Microbiology·Jason F J HuntleyJohn P Bannantine
Sep 2, 2005·Molecular Microbiology·Jason A MacGurnJeffery S Cox
May 31, 2006·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·S Robbe-AustermanJ R Stabel
Dec 6, 2005·Veterinary Microbiology·Mitchell V Palmer, W Ray Waters
Apr 2, 2014·BioMed Research International·Christine GoepfertHorst Posthaus
Aug 12, 2014·BioMed Research International·María Laura MonMaría Isabel Romano
Jun 16, 2012·Veterinary Immunology and Immunopathology·Ramon A Juste
May 18, 2004·Veterinary Microbiology·Elke VansnickFrançoise Portaels
Jul 3, 2009·Clinical and Vaccine Immunology : CVI·Irene SchillerBruno Oesch
Jul 10, 2009·Clinical and Vaccine Immunology : CVI·Irene SchillerBruno Oesch
Jul 17, 2009·Clinical and Vaccine Immunology : CVI·Lawrence R GreenChris V Rathe

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