Use of recombinant human deoxyribonuclease (DNase) for processing of a thawed umbilical cord blood transplant in a patient with relapsed acute lymphoblastic leukemia

Annals of Hematology
Hermann EichlerHarald Klüter

Abstract

This case report describes for the first time the use of a recombinant human enzyme deoxyribonuclease (rhDNase) containing solution for the processing of a thawed umbilical cord blood (UCB) unit prior to successful transplantation to avoid cell losses by clotting phenomena. A 6-year-old boy received an unrelated 2/6 HLA antigen mismatched UCB transplant for high-risk Burkitt type acute lymphoblastic leukemia. The UCB unit was provided as a volume-reduced sample after buffy coat separation with a final volume of 36 ml. To avoid the loss of nucleated cells due to cell clumping during thawing procedure cells were washed with a solution containing the rhDNase. No visible clotting of the resuspended unit occurred, and the patient was transplanted with 2.9x10(7) nucleated cells/kg body weight without any acute or chronic side effects due to rhDNase. On day +35, PCR analysis of bone marrow aspirate showed complete chimerism, and the child engrafted with an absolute neutrophil count greater than 0.5x10(9)/l on day +47. Platelet transfusion independence was achieved on day +120. In conclusion, the supplementation of rhDNase to the washing and resuspension solutions of a thawed UCB unit is effective to prevent cell losses prior to transp...Continue Reading

Citations

Aug 31, 2006·Cell and Tissue Banking·Sajio Sumida
Nov 5, 2003·Journal of Hematotherapy & Stem Cell Research·Pascale DuchezZoran Ivanovic
Jun 6, 2013·ISRN Dentistry·Istabrak HasanFriedhelm Heinemann
Jun 2, 2006·Journal of Immunological Methods·Alfonso J García-PiñeresLigia A Pinto
Feb 17, 2006·Transfusion Medicine·C WoelkeB K Flesch
Feb 4, 2016·Current Protocols in Stem Cell Biology·Megan D HobanDonald B Kohn

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