Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro

Virology Journal
Anthony SimmonsRobert A Davey

Abstract

Genetic redirection of lymphocytes that have been genetically engineered to recognize antigens other than those originally programmed in their germlines is a potentially powerful tool for immunotherapy of cancers and potentially also of persistent viral infections. The basis for this procedure is that both cancers and some viruses have developed strikingly similar mechanisms of evading attacks by host immune mechanisms. To redirect human peripheral blood lymphocytes (PBLs) with a chimeric T cell receptor (chTCR) so that they recognize a new target requires a high degree of transfection efficiency, a process that is regarded as technically demanding. Infection with a retroviral vector carrying a chTCR cassette was shown to transduce 100% of rapidly dividing murine T cells but typically, only approximately 10% of PBLs could be infected with the same vector. In contrast with other retroviruses, lentiviruses integrate their genomes into non-dividing cells. To increase host cell range, vesicular stomatitis virus G protein was pseudotyped with a lentivirus vector, which resulted in approximately 100% PBL transduction efficiency. Signaling of PBLs bearing chimeric receptors was shown by specific proliferation on exposure to cells expr...Continue Reading

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Citations

Mar 26, 2011·Expert Opinion on Biological Therapy·Rabea HinkelChristian Kupatt
Sep 14, 2016·Epidemiology and Infection·A TazeenS Parveen
Feb 25, 2010·Journal of Leukocyte Biology·Jennifer A Westwood, Michael H Kershaw
Jan 16, 2020·Journal of Clinical Medicine·Daniel Sur AlexandruIrimie
Jul 16, 2020·Cancers·Eline JanssenDaniele V F Tauriello
Nov 24, 2020·OncoTargets and Therapy·Xianzhe YuJianguo Li

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Methods Mentioned

BETA
PCR
transfection

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