PMID: 11313126Apr 21, 2001Paper

Use of repetitive DNA elements to define genetic relationships among Anaplasma marginale isolates

FEMS Microbiology Letters
A M FerreiraM C Vidotto

Abstract

Anaplasma marginale genomic DNA was tested for the presence of repetitive extragenic palindromic (REP) and enterobacterial repetitive intergenic consensus (ERIC)-like sequences in order to evaluate the genetic diversity of multiple A. marginale isolates. A. marginale isolates were obtained from cattle of six different states of Brazil, from the US and an Anaplasma centrale strain was obtained from Uruguay. Patterns obtained from A. marginale isolates varied from 14 to 17 fragments by REP-polymerase chain reaction (PCR) and 6 to 14 fragments by ERIC-PCR. All A. marginale isolates presented a 0.75-kb fragment by REP and two common fragments (0.38 and 1.0 kb) by ERIC-PCR. These two fragments were not detectable in A. centrale. Both methods produced similar patterns (80%) among A. marginale isolates obtained from the same region, although some isolates within regions shared less similarity. Isolates from Parana and Pernambuco, were differentiated by these methods. The study demonstrates the presence of ERIC and REP-like elements in A. marginale isolates and shows that A. marginale isolates and strains can be differentiated by these methods.

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Citations

Aug 2, 2002·Veterinary Microbiology·José de la FuenteKatherine M Kocan
May 12, 2004·Veterinary Parasitology·José de La FuenteKatherine M Kocan
Jul 1, 2003·FEMS Microbiology Ecology·Luiz Gustavo Dos Anjos BorgesGertrudes Corção
Oct 26, 2006·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·M C VidottoO Vidotto
May 3, 2013·Parasitology Research·Limei ZhangShujing Wang

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