Use of response surface methodology for optimizing process parameters for high inulinase production by the marine yeast Cryptococcus aureus G7a in solid-state fermentation and hydrolysis of inulin

Bioprocess and Biosystems Engineering
Jun ShengJing Li

Abstract

The optimization of process parameters for high inulinase production by the marine yeast strain Cryptococcus aureus G7a in solid-state fermentation (SSF) was carried out using central composite design (CCD), one of the response surface methodologies (RSMs). We found that moisture, inoculation size, the amount ratio of wheat bran to rice husk, temperature and pH had great influence on inulinase production by strain G7a. Therefore, the CCD was used to evaluate the influence of the five factors on the inulinase production by strain G7a. Then, five levels of the five factors above were further optimized using the CCD. Finally, the optimal parameters obtained with the RSM were the initial moisture 61.5%, inoculum 2.75%, the amount ratio of wheat bran to rice husk 0.42, temperature 29 degrees C, pH 5.5. Under the optimized conditions, 420.9 U g(-1) of dry substrate of inulinase activity was reached in the solid-state fermentation culture of strain G7a within 120 h whereas the predicted maximum inulinase activity of 436.2 U g(-1) of inulinase activity of 436.2 U g(-1) of dry weight was derived from the RSM regression. This is the highest inulinase activity produced by the yeast strain reported so far. A large amount of monosaccharides...Continue Reading

Citations

Jan 6, 2009·Applied Microbiology and Biotechnology·Zhenming ChiLixi Yue
May 19, 2010·Marine Drugs·Sreyashi SarkarJoydeep Mukherjee
Nov 17, 2009·Bioprocess and Biosystems Engineering·Yuling ZhuWen Huang
Nov 28, 2013·Preparative Biochemistry & Biotechnology·M DilipkumarN Rajamohan
Aug 16, 2019·Bioprocess and Biosystems Engineering·Mustafa Germec, Irfan Turhan
Jun 9, 2018·Biotechnology for Biofuels·Yibin QiuPingkai Ouyang

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