Use of rhBMP-2 activated chitosan films to improve osseointegration

Biomacromolecules
José L López-LacombaViviana Ramos

Abstract

Considering the design and development of biomaterials used in tissue engineering, not only is it important that they are biocompatible but also that they induce the desired cellular response for tissue regeneration. Chitosan, a biocompatible and bioresorbable polymer of N-acetylglucosamine and glucosamine is used in our work combined with recombinant human BMP-2 (rhBMP-2), a potentially useful activation factor for bone repair. In this way, we try to combine the biological and filmogenic properties of this biopolymer with the osseoinductive ability of the rhBMP-2. Results showed that the chitosan films employed, without and with rhBMP-2 activation, are able to support cellular growth and proliferation on them and that only the rhBMP-2 activated ones are able to differentiate from a myoblastic mouse cell line (C2C12) toward osteoblastic phenotype. Osseoinduction properties of rhBMP-2 activated films persist for a long storage time. The in vivo experiments performed confirm the expectative created by the in vitro results obtained and are an indication that rhBMP-2 activated chitosan films could be a very attractive biomaterial for the enhancement of osseointegration of surgical prostheses and implants and for the purpose of tiss...Continue Reading

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Citations

Apr 10, 2013·Journal of Biomaterials Science. Polymer Edition·Rusdianto BudiraharjoEn-Tang Kang
May 22, 2008·Tissue Engineering. Part a·Ander AbarrategiJosé Luís López-Lacomba
Jun 4, 2008·Plastic and Reconstructive Surgery·Thomas EngstrandOlle Larm
Oct 5, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Nimitt G PatelPhilip A Yuya
Jan 1, 2010·Cell and Tissue Biology·M I BlinovaG P Pinaev
Apr 4, 2009·Acta Biomaterialia·Ander AbarrategiJosé Luis López-Lacomba
Sep 9, 2008·Journal of Clinical Periodontology·Ronald E JungChristoph H F Hammerle
Sep 17, 2016·AAPS PharmSciTech·Enrique Martínez-CamposInmaculada Aranaz
Jan 23, 2017·International Journal of Biological Macromolecules·Jayachandran VenkatesanMin Suk Shim
Sep 14, 2018·Clinical Implant Dentistry and Related Research·Ulku Tugba KalyoncuogluGulay Kansu
Apr 3, 2008·Journal of Tissue Engineering and Regenerative Medicine·P C BessaR L Reis
Jan 5, 2019·Applied Microbiology and Biotechnology·Muhammad Shahid Riaz RajokaShahid Mahmood
Feb 10, 2021·Journal of Bioscience and Bioengineering·Andrea Y MansillaEnrique Martínez Campos
Jan 1, 2008·Biomacromolecules·Ander AbarrategiJosé Luís López-Lacomba
May 10, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Holly J MartinKeisha B Walters
Jul 10, 2017·ACS Biomaterials Science & Engineering·Ana CivantosAnder Abarrategi

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