Use of robotized DNA isolation and real-time PCR to quantify and identify close correlation between levels of Neisseria meningitidis DNA and lipopolysaccharides in plasma and cerebrospinal fluid from patients with systemic meningococcal disease

Journal of Clinical Microbiology
Reidun ØvstebøPeter Kierulf

Abstract

The present study, using robotized DNA isolation and quantitative PCR based on the Neisseria meningitidis-specific capsular transport A gene, demonstrates the ease, rapidity, specificity, and sensitivity of quantifying neisserial DNA in plasma (n = 65) and cerebrospinal fluid (CSF) (n = 12) from patients with systemic meningococcal disease. We found a close correlation between the levels of neisserial DNA and lipopolysaccharides in plasma (r = 0.905) and in CSF (r = 0.964). The median concentration of neisserial DNA in plasma in 23 patients with persistent shock was 2 x 10(7) copies/ml, versus <10(3) copies/ml in 42 nonshock patients. Furthermore, quantitative PCR made possible estimates of the total number of meningococci in plasma, as opposed to conventional blood cultures, suggesting about 1,000 dead meningococci for every viable bacterium. Finally, with logistic regression analyses, neisserial DNA may predict a patient's disease severity and outcome at hospital admission. The number of meningococci in plasma and CSF appears to be the main determinant of the lipopolysaccharide levels, clinical presentation, and outcome.

References

Feb 1, 1989·The Journal of Infectious Diseases·P BrandtzaegE Sørensen
Jan 1, 1987·Scandinavian Journal of Infectious Diseases·B M AndersenK Bøvre
Nov 1, 1973·The Journal of Experimental Medicine·I W Devoe, J E Gilchrist
Mar 1, 1981·Journal of Clinical Microbiology·L J La ScoleaE Neter
Nov 5, 1997·Applied and Environmental Microbiology·I G Wilson
Jan 11, 2000·Clinical Microbiology Reviews·M van DeurenJ W van der Meer
Dec 26, 2001·Journal of Endotoxin Research·P BrandtzaegP Kierulf
Jan 25, 2002·Archives of Disease in Childhood·S J HackettC A Hart
Dec 14, 2002·Lancet·Ellen Namork, Petter Brandtzaeg
Oct 15, 2003·FEMS Immunology and Medical Microbiology·Georgina TzanakakiJenny Kremastinou
Oct 16, 2004·Expert Review of Anti-infective Therapy·Petter Brandtzaeg
Jan 1, 2001·Methods in Molecular Medicine·P Olcén, H Fredlund
Jan 1, 2001·Methods in Molecular Medicine·M Guiver, R Borrow
Jan 1, 2001·Methods in Molecular Medicine·M A SobanskiW T Coakley
Jan 1, 2001·Methods in Molecular Medicine·S J GrayE B Kaczmarski
Jan 1, 2001·Methods in Molecular Medicine·P BrandtzaegP Kierulf

❮ Previous
Next ❯

Citations

May 22, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S C de GreeffM van Deuren
Feb 11, 2011·Current Infectious Disease Reports·Anand Kumar
Sep 16, 2010·Current Treatment Options in Neurology·Ricardo G Branco, Robert C Tasker
Jul 11, 2013·The Journal of Infectious Diseases·Olivier Join-LambertXavier Nassif
Aug 13, 2005·Critical Care Medicine·Marcel van Deuren, Petter Brandtzaeg
Jul 2, 2008·Infection and Immunity·Bernt Christian HellerudPetter Brandtzaeg
Dec 19, 2007·Infection and Immunity·Robert S Munford
Sep 18, 2012·Journal of Bacteriology·Elena Del TordelloDavide Serruto
May 30, 2008·Journal of Clinical Microbiology·Martin J CallaghanAndrew J Pollard
Jul 9, 2013·Critical Care Clinics·Paul A CampsallDaniel J Niven
Jul 3, 2007·Lancet·David S StephensPetter Brandtzaeg
Dec 7, 2006·The Journal of Infection·Hamish M LalaDiana Martin
Nov 30, 2012·Cellular Microbiology·Florence MillerMathieu Coureuil
Aug 30, 2005·Advances in Pediatrics·Petter Brandtzaeg, Marcel van Deuren
Feb 14, 2014·Journal of Medical Microbiology·Christian LeliAntonella Mencacci
Jan 7, 2015·Journal of Veterinary Emergency and Critical Care·Iain Keir, Amy E Dickinson
Jan 20, 2007·FEMS Microbiology Reviews·Simon Nadel, J Simon Kroll
Jan 20, 2007·FEMS Microbiology Reviews·David S Stephens
Jun 3, 2015·Current Infectious Disease Reports·Stephen Y Liang, Anand Kumar
Sep 6, 2014·Frontiers in Cellular and Infection Microbiology·Christoph SchoenBiju Joseph Ampattu
Jun 4, 2016·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Ivano de FilippisAntonio Eugenio de Almeida
Nov 4, 2017·Virulence·Elena CapelMathieu Coureuil
Aug 19, 2017·Critical Care Medicine·Andre C Kalil

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.