May 4, 1978

Use of salicylic acid to measure the apparent intracellular pH in the Ehrlich ascites-tumor cell and Escherichia coli

Biochimica Et Biophysica Acta
J Garcia-Sancho, A Sanchez

Abstract

The distribution of salicylic acid between the intracellular and extracellular phases has been used to estimate the intracellular pH in the Ehrlich cell and Escherichia coli. The validity of the method was established by: (i) comparison of the results obtained with salicylic acid with those obtained with 5,5-dimethyloxazolidine-2,4-dione; (ii) by following changes of the apparent intracellular pH under circumstances in which such changes are predictable, e.g., the addition of weak acids or proton conductors to the incubation medium during incubation at acidic pH; (iii) by comparison of the apparent intracellular pH changes with the uptake of H+ by the cells estimated from the changes of the medium pH. Optimal results are obtained with this indicator when the extracellular pH is below 5.5, because in this case the indicator is to a sufficient extent in its penetrating form, so that its movement can reflect intracellular pH changes occurring in less than 30 s. When the intracellular pH falls below 5.2 measurable binding of salicylic acid to the intracellular material of the Ehrlich cell takes place, but above this pH no binding has been found. The Ehrlich cell and cells of Escherichia coli behaved similarly under various experime...Continue Reading

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References

Mentioned in this Paper

Tumor Cells, Uncertain Whether Benign or Malignant
Dinitrophenols
Alkalescens-Dispar Group
Extracellular
Neoplastic Cell
Protoplasm
Uptake
Salicylic Acid
Carcinoma, Ehrlich Tumor
Acids

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