Use of Self-Assembling Peptides to Enhance Stem Cell Function for Therapeutic Angiogenesis

Stem Cells International
Hyung Sub ParkTaeseung Lee

Abstract

The use of nanomaterials for biomedical applications has become a promising field in regenerative medicine. Self-assembling peptides (SAPs) have been proposed as a good candidate because they are able to self-assemble into stable hydrogels and interact with cells or molecules when combined together. This in turn can lead to the improved survival or action of cells or molecules to obtain the desired effects. In this study, we investigated whether the combination of mesenchymal stem cells (MSCs) with SAPs could improve angiogenesis in ischemic hindlimbs of rats compared to MSC or SAP treatment alone. The combination of MSCs and SAPs showed an overall higher expression of angiogenesis markers on fluorescent immunohistochemical analysis and a lower degree of fibrosis and cell apoptosis, which in turn led to an overall tendency for improved perfusion of the ischemic hindlimbs. Finally, SAPs also showed the ability to recruit endogenous host MSCs into the site of action, especially when modified to incorporate substance P as a functional motif, which when injected with exogenous MSCs, allowed for the dual presence of MSCs at the site of action. Overall, these results suggest that SAPs can be applied with stem cells to potentiate angi...Continue Reading

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Citations

Nov 5, 2019·Current Stem Cell Research & Therapy·L Vinod Kumar ReddyDwaipayan Sen
Apr 11, 2020·Tissue Engineering. Part B, Reviews·Jianrui Ma, Chengyang Huang
Nov 4, 2020·Free Radical Biology & Medicine·Malgorzata KucinskaMarek Murias

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Methods Mentioned

BETA
amputation
biosensor
Assay
ELISA
PCR

Software Mentioned

IncuCyte system
SPSS Statistics

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