Use of simulated intestinal fluids with Caco-2 cells and rat ileum

Drug Development and Industrial Pharmacy
N PatelJ Murray

Abstract

In most in vitro studies of oral drug permeability, little attempt is made to reproduce the gastrointestinal lumenal environment. The aim of this study was to evaluate the compatibility of simulated intestinal fluid (SIF) solutions with Caco-2 cell monolayers and Ussing chamber-mounted rat ileum under standard permeability experiment protocols. In preliminary experiments, fasted-state simulated intestinal fluid (FaSSIF) and fed-state simulated intestinal fluid (FeSSIF) solutions based on the dissolution medium formulae of Dressman and co-workers (1998) were modified for compatibility with Caco-2 cells to produce FaS-SIF and FeSSIF "transport" solutions for use with in vitro permeability models. For Caco-2 cells exposed to FaSSIF and FESSIF transport solutions, the transepithelial electrical resistance was maintained for over 4 h and mannitol permeability was equivalent to that in control (Hank's Balanced Salt Solution-treated) cell layers. Scanning electron microscopy revealed that microvilli generally maintained a normal distribution, although some shortening of microvilli and occasional small areas of denudation were observed. For rat ileum in the Ussing chambers, the potential difference (PD) collapsed to zero over 120 min w...Continue Reading

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Citations

Feb 19, 2013·Advanced Drug Delivery Reviews·Jennifer M Gamboa, Kam W Leong
Dec 17, 2014·Drug Development and Industrial Pharmacy·Sumon ChakrabortyDeepika Aggarwal
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Feb 4, 2021·Pharmaceutics·Glenn LemmensPatrick Augustijns
Jan 16, 2018·ACS Biomaterials Science & Engineering·Wenda ZhouDavid L Kaplan

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