Use of Sno Strip filter-paper wicks for collection of genital-tract samples allows reproducible determination of human immunodeficiency virus type 1 (HIV-1) RNA viral load with a commercial HIV-1 viral load assay

Journal of Clinical Microbiology
Christopher H SherlockDavid R Burdge

Abstract

To assess the reproducibility of measurements of cervical and vaginal human immunodeficiency virus (HIV) viral load, 92 duplicate cervical and 88 duplicate vaginal samples were collected from 13 HIV-infected women using Sno Strip filter-paper wicks. RNA was eluted from the strips, extracted, and assayed using a modified protocol for the Roche Cobas Amplicor HIV-1 Monitor assay. Pearson's correlation coefficient (R), coefficient of determination (D), and Bland-Altman plots (BA) were used to compare paired log10-transformed viral loads. Analysis of duplicate same-site samples showed good reproducibility (cervix: R = 0.72, D = 52%, BA = 89% within range; vagina: R = 0.72, D = 51%, BA = 87% within range); paired cervix/vagina measurements showed moderate correlation only (R = 0.56; D = 31.3%). Standardized sample collection and simple modification of the Roche Cobas Amplicor HIV-1 Monitor assay allows reproducible measurement of genital viral load.

References

Apr 16, 1998·The Journal of Infectious Diseases·C GoulstonD Katzenstein
Mar 9, 1999·The Journal of Infectious Diseases·C E HartT V Ellerbrock
Apr 4, 2000·Journal of Clinical Microbiology·R N ShepardS A Fiscus
Mar 10, 2001·The Journal of Infectious Diseases·C C WangJ K Kreiss
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Aug 21, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S Cu-UvinUNKNOWN HIV Epidemiology Research Study

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Citations

Jan 2, 2007·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Andrea KunzGundel Harms
Dec 19, 2009·Pharmaceutical Development and Technology·David R Friend
May 26, 2018·The Journal of Antimicrobial Chemotherapy·Shanmugam SaravananRami Kantor

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