Use of solid-phase extraction to determine ergosterol concentrations in plant tissue colonized by fungi.

Applied and Environmental Microbiology
M O Gessner, A L Schmitt

Abstract

At present, the ergosterol and acetate-to-ergosterol techniques are generally considered to be the methods of choice to quantify fungal biomass, growth rate, and productivity under natural conditions. Both methods rely on the accurate isolation and quantification of ergosterol, a major membrane component of eumycotic fungi. Taking advantage of the solid-phase extraction (SPE) technique, we present a novel method to determine the ergosterol concentration in lipid extracts derived from plant tissues and dead organic matter colonized by fungi. In this method, a primary alkaline extract is acidified and passed through a reversed-phase (C(inf18)) SPE column. The column is then washed with an alkaline methanol-water solution to eliminate interfering substances and increase pH and is thoroughly dried in air. Ergosterol is eluted with alkaline isopropanol. This eluting solvent was chosen to produce a strongly basic pH of the final extract and thus confer stability on the ergosterol molecule before high-performance liquid chromatography analysis. The recovery of ergosterol from plant tissues and the O(infhf) horizon of a woodland soil ranged from 85 to 98%, and the overall extraction efficiency was similar to that obtained by a conventi...Continue Reading

References

Sep 1, 1988·Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung·G ZillP R Wallnöfer
Feb 1, 1993·Applied and Environmental Microbiology·M O Gessner, E Chauvet

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Citations

Jul 6, 2010·Applied and Environmental Microbiology·Christoph TellenbachThomas N Sieber
Sep 11, 2012·Environmental Microbiology·Aline FrossardMark O Gessner
Nov 6, 2015·The New Phytologist·Joseph J HendricksStephen D Pecot
Sep 4, 2004·Applied and Environmental Microbiology·Cláudia Pascoal, Fernanda Cássio
Jun 15, 2006·The New Phytologist·Joseph J HendricksStephanie E Sims
Dec 15, 2010·Applied and Environmental Microbiology·Sabine Flury, Mark O Gessner
Aug 20, 2009·Ecological Applications : a Publication of the Ecological Society of America·Mary J HarnerRobert L Sinsabaugh
Jan 5, 2006·Applied and Environmental Microbiology·Nanna Buesing, Mark O Gessner
Aug 15, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·H MiyagawaT Goto
Mar 5, 2005·Applied and Environmental Microbiology·Brian BoyleArmand Séguin
Sep 16, 2011·Environmental Toxicology and Chemistry·Jochen P ZubrodRalf Schulz
Sep 13, 2016·Frontiers in Microbiology·Gwenaëlle LashermesIsabelle Bertrand
Mar 4, 2010·Proteomics·Thomas SchneiderKathrin Riedel
Aug 14, 2018·FEMS Microbiology Ecology·Jérémy JabiolMark O Gessner
Feb 8, 2014·The Science of the Total Environment·Miguel Cañedo-ArgüellesRalf B Schäfer
May 16, 2021·Fungal Genetics and Biology : FG & B·Benoit LaurentMarie Foulongne-Oriol
Mar 22, 2000·Journal of Agricultural and Food Chemistry·F de SioL Servillo
Jun 8, 2006·Journal of Agricultural and Food Chemistry·Yanhong DongChester J Mirocha

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