PMID: 6401795Feb 1, 1983Paper

Use of somatic cell genetics to study chromosomes contributing to antigen plus I recognition by T cell hybridomas

The Journal of Experimental Medicine
P Marrack, J Kappler

Abstract

Keyhole limpet hemocyanin (KLH)/I region-specific T cell hybridomas have been prepared by fusing KLH/I-specific T cell blasts from mice with single pairs of metacentric chromosomes to the inducible, interleukin 2 (IL-2)-secreting T cell hybridoma FS6-14.13.AG2.1. T cell hybridomas with KLH/I receptors were identified by their ability to secrete IL-2 in response to KLH and the appropriate antigen-presenting cells. After cloning and subcloning, KLH/I reactivity was correlated with the presence or absence of metacentric chromosomes derived from the KLH/I-specific T cell blast parent. Hybridomas were identified that had lost all chromosomes 4 and 6 or 16 and 17 derived from their normal T cell parent, but retained the ability to respond to KLH/I. This suggested that products of genes on these chromosomes did not contribute to the specific portions of T cell Ag/I receptors. These gene products would include, of course, kappa and lambda chains and H-2. We did not obtain any T cell hybridomas that had lost both metacentric (8.12) chromosomes derived from T cells of the Robertsonian mouse strain Rb(8.12)5, so we could not draw any conclusions about the contributions of products of genes on these chromosomes. T cell hybridomas with KLH/...Continue Reading

References

Jan 1, 1976·Scandinavian Journal of Immunology·C A JanewayH Binz
Oct 1, 1978·The Journal of Experimental Medicine·G K Lewis, J W Goodman
Feb 1, 1979·The Journal of Immunology : Official Journal of the American Association of Immunologists·K J KimR Asofsky
Jul 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J Klein
Jan 1, 1980·Molecular Immunology·R L WilderR G Mage
Oct 1, 1980·The Journal of Experimental Medicine·E A LernerD B Murphy
Jul 1, 1982·The Journal of Experimental Medicine·L A Sherman
Oct 1, 1980·The Journal of Experimental Medicine·L HarwellJ Kappler
Jan 1, 1982·The Journal of Experimental Medicine·T R Hünig, M J Bevan
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J KapplerP Marrack
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D DavignonT A Springer
Oct 1, 1975·European Journal of Immunology·K Eichmann, K Rajewsky

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Citations

Sep 1, 1984·The Journal of Experimental Medicine·N E LeeM M Davis
Aug 1, 1986·Kidney International·E G NeilsonR Mann
May 1, 1983·Immunology Today·J F MillerI D Walker
Jan 1, 1983·Immunological Reviews·P MarrackJ Kappler

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