Use of soybean peroxidase in chemiluminescent enzyme-linked immunosorbent assay

Journal of Agricultural and Food Chemistry
Ivan Yu SakharovEvgeny E Efremov

Abstract

A procedure for the production of conjugates of soybean peroxidase (SbP) oxidized by sodium periodate and anti-mouse IgG antibody (Ab) was optimized. A sandwich chemiluminescent enzyme-linked immunosorbent assay (ELISA) for determination of mouse IgG using SbP and specific Ab was developed, and SbP-catalyzed oxidation of luminol was carried out in the absence of any enhancer. Comparison of conjugates produced by labeling Ab by soybean and horseradish peroxidases in the chemiluminescent ELISA showed that in the case of SbP a rate of emission decay formed through luminol oxidation was significantly lower. Application of the soya enzyme allowed the development of the immunoassay with improved sensitivity and a wider linear range.

Citations

May 12, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Aiping FanJianzhong Lu
May 8, 2010·Biotechnology Journal·Marina M VdovenkoIvan Y Sakharov
Jul 4, 2006·Trends in Biotechnology·Barry J RyanCiarán O'Fágáin
Aug 19, 2015·Analytical and Bioanalytical Chemistry·Laura ZehnackerStéphane Azoulay
Aug 26, 2014·Analytical Biochemistry·Anastasia V GribasIvan Yu Sakharov
Nov 28, 2007·Prikladnaia biokhimiia i mikrobiologiia·A N BerlinaI Iu Sakharov

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