Use of stable isotopes to study fatty acid and lipoprotein metabolism in man

Prostaglandins, Leukotrienes, and Essential Fatty Acids
J T Brenna

Abstract

Tracer studies have long been an important tool for lipid metabolism research. Recent advances and availability of high performance mass spectrometers (MS) and improved stable isotopically labeled tracers contribute to an increase in stable isotope tracer studies in humans. We briefly review recent studies and discuss advances in high sensitivity methods and applications. GC/MS analysis. Tracer studies with gas chromatography/mass spectrometry (GC/MS) usually rely on D labeling, where labeling with more that three D atoms shifts the analyte mass above that of the natural abundance envelope, and the MS monitors selected masses representing the isotopimers of interest. Recent examples are the work of Emken and coworkers, who investigated the desaturation of 18:0 and 16:0, and 18:2n-6 and 18:3n-3 elongation/desaturation, in adults, with oral doses of about 3 g of d2,4,6 fatty acids. They showed modest levels of 18:0 and 16:0 desaturation over 2 days and an influence of dietary 18:2n-6 on elongation. In premature infants, Salem, Uauy and coworkers recently have used d5-18:2n-6 and d5-18:3n-3 doses of 50-100 mg/kg body weight to show that infants as small as 1980 g and 32 weeks gestation elongate and desaturate both precursors withi...Continue Reading

References

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Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·N SalemR Uauy
Jul 1, 1995·Journal of Pediatric Gastroenterology and Nutrition·H DemmelmairB Koletzko
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May 1, 1994·Journal of the American Society for Mass Spectrometry·D A Merritt, J M Hayes

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Citations

Apr 16, 1998·Mass Spectrometry Reviews·J T BrennaR J Caimi
Aug 16, 2003·Biomolecular Engineering·Francisco G Acién FernándezEmilio Molina Grima
May 2, 2013·The British Journal of Nutrition·R Chouinard-WatkinsM Plourde
Feb 1, 2007·Analytical Chemistry·Parminder Kaur, Peter B O'Connor
Mar 11, 2000·Current Opinion in Clinical Nutrition and Metabolic Care·W Meier-Augenstein
Jan 29, 2011·Nutrition & Metabolism·Mélanie PlourdeStephen C Cunnane
Oct 6, 2006·Journal of Lipid Research·Sébastien GagnéJean-François Lévesque
Dec 21, 2014·The American Journal of Clinical Nutrition·Joseph J LeeElizabeth J Parks
May 16, 2014·The American Journal of Clinical Nutrition·Mélanie PlourdeStephen C Cunnane
Sep 25, 2004·Mass Spectrometry Reviews·Gregory G DolnikowskiFrancine K Welty
Jan 3, 2019·Angewandte Chemie·Tiffany Porta SiegelShane R Ellis

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