Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of acanthamoebae from humans with keratitis and from sewage sludge

Journal of Clinical Microbiology
J M SchroederThomas J Byers

Abstract

This study identified subgenic PCR amplimers from 18S rDNA that were (i) highly specific for the genus Acanthamoeba, (ii) obtainable from all known genotypes, and (iii) useful for identification of individual genotypes. A 423- to 551-bp Acanthamoeba-specific amplimer ASA.S1 obtained with primers JDP1 and JDP2 was the most reliable for purposes i and ii. A variable region within this amplimer also identified genotype clusters, but purpose iii was best achieved with sequencing of the genotype-specific amplimer GTSA.B1. Because this amplimer could be obtained from any eukaryote, axenic Acanthamoeba cultures were required for its study. GTSA.B1, produced with primers CRN5 and 1137, extended between reference bp 1 and 1475. Genotypic identification relied on three segments: bp 178 to 355, 705 to 926, and 1175 to 1379. ASA.S1 was obtained from single amoeba, from cultures of all known 18S rDNA genotypes, and from corneal scrapings of Scottish patients with suspected Acanthamoeba keratitis (AK). The AK PCR findings were consistent with culture results for 11 of 15 culture-positive specimens and detected Acanthamoeba in one of nine culture-negative specimens. ASA.S1 sequences were examined for 6 of the 11 culture-positive isolates and ...Continue Reading

References

Jul 1, 1990·The Journal of protozoology·T J ByersV J Stewart
Nov 1, 1990·The Journal of protozoology·K Yagita, T Endo
Mar 1, 1991·Reviews of Infectious Diseases·G S Visvesvara
Jul 1, 1989·Computer Applications in the Biosciences : CABIOS·E L Cabot, A T Beckenbach
Jan 1, 1988·Parasitology Research·J F De Jonckheere, R Michel
Jan 1, 1986·International Review of Cytology·T J Byers
May 1, 1995·Molecular and Biochemical Parasitology·R J Gast, T J Byers
Apr 1, 1994·Journal of Clinical Microbiology·R K GautomT R Fritsche
Jul 1, 1993·The Journal of Eukaryotic Microbiology·G S VisvesvaraA J Martinez
Jan 1, 1997·Brain Pathology·A J Martinez, G S Visvesvara
Jun 25, 1998·The Korean Journal of Parasitology·D I ChungH H Kong
Jan 8, 2000·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J M AlvesH T Affonso
Nov 24, 2005·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·D V SealUNKNOWN Keratitis Study Group

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Citations

Jul 19, 2006·Archives of Microbiology·Abdul MatinNaveed Ahmed Khan
Oct 21, 2009·Current Microbiology·Ana Maris CarlessoMarilise Brittes Rott
Sep 2, 2011·Current Microbiology·Mari Aline Todero WinckMarilise Brittes Rott
Oct 3, 2006·Parasitology Research·Hatice ErtabaklarJulia Walochnik
Jun 30, 2009·Parasitology Research·Windell L Rivera, Davin Edric V Adao
Apr 23, 2010·Parasitology Research·Daniele Corsaro, Danielle Venditti
Mar 3, 2011·Parasitology Research·Zohreh LasjerdiEhsan Nazemalhosseini Mojarad
Jul 13, 2011·Parasitology Research·Mohammad Reza MahmoudiPanagiotis Karanis
Mar 30, 2007·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H YeraC Chaumeil
Dec 19, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D Di CaveF Berrilli
Jun 21, 2005·International Journal for Parasitology·Marian L TurnerKeryn A Williams
Jun 1, 2005·Journal of Microbiological Methods·Delphine RivièreYann Héchard
Nov 10, 2013·Water Research·A MagnetC Del Aguila
Jan 23, 2009·Cornea·Simon HaslerMichael A Thiel
Sep 9, 2008·Applied and Environmental Microbiology·Miriam EddyaniFrançoise Portaels
Apr 15, 2003·Clinical Microbiology Reviews·Francine Marciano-Cabral, Guy Cabral
Nov 19, 2008·Infection and Immunity·A MattanaP Cappuccinelli
Jun 4, 2010·Journal of Clinical Microbiology·Céline CoulonVincent Thomas
May 1, 2009·Journal of Clinical Microbiology·Stephanie P JohnstonGovinda Visvesvara
Oct 6, 2006·Journal of Clinical Microbiology·Yvonne QvarnstromAlexandre J da Silva
Oct 17, 2008·Journal of Clinical Microbiology·Rama SriramGovinda S Visvesvara
Mar 27, 2009·Journal of Clinical Microbiology·D R LedeeE C Alfonso
Jan 9, 2003·Journal of Clinical Microbiology·Gregory C BootonPaul A Fuerst
Jul 5, 2003·Journal of Clinical Microbiology·Gunisha PasrichaRamesh K Aggarwal
Apr 9, 2005·Journal of Clinical Microbiology·Gregory C BootonPaul A Fuerst

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