Use of supF, an Escherichia coli tyrosine suppressor tRNA gene, as a mutagenic target in shuttle-vector plasmids

Mutation Research
K H Kraemer, M M Seidman

Abstract

The Escherichia coli tyrosine amber suppressor tRNA gene, supF, has been utilized as a mutagenic target in several shuttle-vector plasmids. Data on mutagenic inactivation of suppressor activity was obtained from induced mutagenesis experiments with plasmids pZ189 and p3AC, and from studies on alterations of the supF gene transduced into E. coli. 162 single or tandem base-substitution mutations that reduce or eliminate suppressor activity were identified at 86 sites within 158 base pairs. The 2 transition and 4 transversion mutations possible in double-stranded DNA were all detectable. At 56 sites two different inactivating mutations were found; and at 20 sites all 3 possible base substitution mutations inactivated suppressor function. Most of the mutations were clustered within the mature tRNA region: 144 of the base-substitution mutations were found at 74 sites within the 85-bp mature tRNA region. Insertions of 1 or 2 bases at 4 sites and deletions of 1 to 3 bases at 15 sites were found to inactivate supF function. A few silent mutations which do not inactivate suppressor function were found: single base-substitutions at 4 sites, 14 pairs of silent double mutations, and a large deletion including the promoter region. The supF ...Continue Reading

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Citations

Jun 3, 1994·Molecular & General Genetics : MGG·S Akasaka, K Yamamoto
Oct 25, 2013·Molecular Genetics and Genomics : MGG·Keiichiro HiratsuTatsuo Nunoshiba
Jun 7, 2005·Chemico-biological Interactions·Margaret GaskellPeter B Farmer
Jun 6, 2000·Mutation Research·K A Canella, M M Seidman
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Jun 18, 2005·Nucleic Acids Research·Jennifer M KalishPeter M Glazer
Dec 10, 2009·Molecular and Cellular Biology·George-Lucian MoldovanAlan D D'Andrea

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