Use of synthetic oligodeoxynucleotide probes for the isolation of a human cholinesterase cDNA clone

Journal of Neuroscience Research
C A ProdyH Soreq

Abstract

Cholinesterases are serine esterases that rapidly hydrolyze the neurotransmitter acetylcholine. In humans, cholinesterases exhibit extensive polymorphism in terms of their substrate specificity, sensitivity to selective inhibitors, hydrophobicity, and cellular as well as subcellular localization. It is not yet known whether the various cholinesterase forms originate from different genes or are products of posttranscriptional and posttranslational processing. The extent to which these enzyme forms are homologous in their amino acid sequence is also not known. However, a consensus organophosphate-binding hexapeptide sequence Phe-Gly-Glu-Ser-Ala-Gly was found both in "true" acetylcholinesterase from the electric organ of Torpedo [McPhee-Quigley et al: J Biol Chem 260:12185-12189, 1985] and in "pseudocholinesterase" (butyrylcholinesterase) from human serum [Lockridge: "Cholinesterases--Fundamental and Applied Aspects." New York: de Gruyter pp 5-12, 1984], suggesting that this region in the protein is conserved in all cholinesterases. Based on this common sequence, we prepared synthetic oligodeoxynucleotides and used them as labeled probes to screen a cDNA library from fetal human brain mRNA, cloned in lambda gt10 phages. A cDNA clo...Continue Reading

References

Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M VignyJ Massoulié
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W I WoodR M Lawn
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·H SoreqP Spierer
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·Y TakahashiK Matsubara
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S V SuggsK Itakura
Jan 1, 1982·Annual Review of Neuroscience·J Massoulié, S Bon
Jan 1, 1983·Methods in Enzymology·J Messing
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H SoreqI Silman
Jan 1, 1982·DNA·H R ChenB C Orcutt
Dec 11, 1981·Science·F Sanger
Jan 1, 1981·Immunogenetics·A A ReyesR B Wallace
Jul 1, 1953·The Biochemical Journal·L AUSTIN, W K BERRY

❮ Previous
Next ❯

Citations

Sep 1, 1989·Human Genetics·M L MarazitaM Crist
Feb 1, 1991·Cellular and Molecular Neurobiology
Feb 1, 1991·Cellular and Molecular Neurobiology·A GnattH Soreq
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C A ProdyH Soreq
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C McTiernanO Lockridge
Jan 1, 1994·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·H SoreqH Zakut
Dec 30, 1987·European Journal of Biochemistry·I Silman, A H Futerman
Jan 1, 1987·Molecular Neurobiology·H Soreq, A Gnatt

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.