PMID: 7580798Nov 1, 1995Paper

Use of synthetic peptides to identify surface-exposed, linear B-cell epitopes within outer membrane protein F of Pseudomonas aeruginosa

Current Microbiology
Harry E GillelandJohn Staczek

Abstract

In a previous study (Hughes EE, Gilleland LB, Gilleland HE Jr. [1992] Infect Immun 60:3497-3503), ten synthetic peptides were used to test for surface-exposed antigenic regions located throughout the length of outer membrane protein F of Pseudomonas aeruginosa. An additional nine peptides of 11-21 amino acid residues in length were synthesized. Antisera collected from mice immunized with each of the 19 synthetic peptides conjugated to keyhole limpet hemocyanin were used to determine which of the peptides had elicited antibodies capable of reacting with the surface of whole cells of the various heterologous Fisher-Devlin immunotypes of P. aeruginosa. Cell surface reactivity was measured by an enzyme-linked immunosorbent assay (ELISA) with whole cells of the various immunotypes as the ELISA antigens and by opsonophagocytic uptake assays with the various peptide-directed antisera, immunotype 2 P. aeruginosa cells, and polymorphonuclear leukocytes of human and murine origin. Three peptides located in the carboxy-terminal portion of protein F elicited antibodies with the greatest cell-surface reactivity. Peptide 9 (TDAYNQKLSERRAN), peptide 10 (NATAEGRAINRRVE), and peptide 18 (NEYGVEGGRVNAVG) appear to have sufficient potential for f...Continue Reading

References

Sep 1, 1991·Infection and Immunity·P van der LeyJ T Poolman
Jul 1, 1990·Molecular Microbiology·R E HancockN Martin
Apr 1, 1985·European Journal of Clinical Microbiology·R E HancockE C Mouat
Dec 1, 1986·Journal of Clinical Pathology·R C MatthewsS Tabaqchali
Dec 1, 1982·The Journal of Infectious Diseases·L M MuthariaR E Hancock
Jan 1, 1995·Infection and Immunity·E G RawlingR E Hancock
Oct 1, 1993·Molecular Microbiology·R S WongR E Hancock
Nov 1, 1993·International Journal of Peptide and Protein Research·M M Gromiha, P K Ponnuswamy
Jan 1, 1987·Immunology Today·M W Steward, C R Howard
Oct 1, 1993·Molecular Microbiology·Rebecca S Y WongRobert E W Hancock

❮ Previous
Next ❯

Citations

Apr 6, 2006·The Journal of Biological Chemistry·Etsuko SugawaraHiroshi Nikaido
Mar 12, 2005·Human Gene Therapy·Julie L BoyerRonald G Crystal
Mar 1, 1997·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J DuchateauL Baras
Jul 16, 2005·Expert Opinion on Biological Therapy·E Sedlak-WeinsteinA R Foxwell
Apr 21, 2005·The Journal of Clinical Investigation·Stefan WorgallRonald G Crystal
Jul 2, 2003·FEMS Immunology and Medical Microbiology·Erfan MansouriBernd Ulrich von Specht
Jul 2, 2003·FEMS Immunology and Medical Microbiology·J StaczekH E Gilleland
Jan 8, 2009·The Korean journal of laboratory medicine·Jeong-Su ParkEui-Chong Kim
May 12, 2021·Life Science Alliance·Géraldine MayeuxJean-Luc Lenormand

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.