PMID: 326797Jun 1, 1977Paper

Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy

The Journal of Cell Biology
M H Heggeness, J F Ash

Abstract

A new indirect method for fluorescence localization of proteins making use of the avidin-biotin complex is described. We have prepared both a biotin-modified rabbit heavy meromyosin (BHMM) and a biotin-modified antibody to a smooth muscle myosin. After fixation, cells can be treated with either BHMM, which binds to actin, or the biotinyl antibody, which binds to myosin. In a second step the cell are treated with a fluorescent derivative of avidin (Fl-avidin) which binds to the biotinyl proteins and thus indirectly reveals the location of the cellular action or myosin.

References

Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·K WangS J Singer
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J W Sanger
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H Heitzmann, F M Richards
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·E Lazarides, K Weber
May 28, 1969·Journal of Molecular Biology·S LoweyH Baker
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·K Weber, U Groeschel-Stewart
Feb 1, 1966·Biochemistry·Y TonomuraM Morales

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Citations

Apr 1, 1991·Journal of Cellular Physiology·A A BogdanovV P Torchilin
Jan 1, 1978·Journal of Supramolecular Structure·K E Meier, A E Ruoho
Jan 1, 1978·Journal of Supramolecular Structure·S J SingerD Louvard
Jan 1, 1979·Journal of Supramolecular Structure·R G Painter, A T McIntosh
Mar 1, 1983·The Histochemical Journal·F T Bosman
Apr 1, 1992·Gastroenterologia Japonica·K SakaguchiT Tsuji
Jan 1, 1994·Chemistry and Physics of Lipids·W J McConathyC C Corder
Jun 1, 1982·Developmental Biology·J W FristromD J Silvert
Feb 25, 1983·Journal of Immunological Methods·P V Subba RaoD D Metcalfe
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·L Y Bourguignon, S J Singer
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J F AshS J Singer
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M H HeggenessS J Singer
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M H HeggenessS J Singer
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M H GinsbergE F Plow
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D Louvard
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P R LangerD C Ward
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·W GodfreyL Wofsy
Jan 1, 1992·Critical Reviews in Food Science and Nutrition·S S GazzazF M Dong
Feb 1, 1982·The Journal of Cell Biology·R G Painter, M Ginsberg
Feb 1, 1987·The Journal of Experimental Medicine·D C Reason
Jun 20, 1978·Annals of the New York Academy of Sciences·M H HeggenessS J Singer
Jan 1, 1991·Journal of Enzyme Inhibition·F S StevenH Maier
Jan 1, 1988·Scandinavian Journal of Rheumatology. Supplement·T E MichaelsenP I Gaarder
Oct 13, 2015·Nature Nanotechnology·Fabian BaumannDiana A Pippig
Jul 29, 2008·Journal of Microbiological Methods·Luís Antonio PeroniDagmar Ruth Stach-Machado

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