Use of the cefepime-clavulanate ESBL Etest for detection of extended-spectrum beta-lactamases in AmpC co-producing bacteria

Journal of Infection in Developing Countries
Srujana MohantyMonorama Deb

Abstract

Extended-spectrum beta-lactamases (ESBLs) may not always be detected in routine susceptibility tests. This study reports the performance of the cefepime-clavulanate ESBL Etest for the detection of ESBLs in Enterobacteriaceae, including those producing AmpC enzyme. Consecutive non-duplicate isolates of Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis isolated from bloodstream infections from January to June 2008 were tested for ESBL by both the standard CLSI double-disk diffusion method using ceftazidime and cefotaxime disks and Etests using ceftazidime/ceftazidime-clavulanate, cefotaxime/cefotaxime-clavulanate and cefepime/cefepime-clavulanate gradients. Isolates were also tested for the presence of transferable AmpC beta-lactamase by AmpC disk test and the efficacies of the different Etests in detecting ESBL production were compared. A total of 113 bacterial isolates (61 K. pneumoniae, 50 E. coli, and 2 P. mirabilis) were recovered. Respectively, 42 (37.2%) and 55 (48.7%) isolates were positive for ESBL by the ceftazidime-clavulanate and cefotaxime-clavulanate combined disk tests. The cefepime/cefepime-clavulanate Etest strip detected the maximum number of isolates (70/113, 61.9 %) as ESBL-positive compared to th...Continue Reading

Citations

Jun 19, 2013·BMC Microbiology·Charu KothariMonorama Deb
Nov 9, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E WillemsJ Verhaegen
Nov 3, 2011·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Keite da Silva Nogueira-MirandaLibera Maria Dalla-Costa
Dec 31, 2019·International Journal of Microbiology·Shahzad MirzaNikunja Kumar Das
Jan 17, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Benoît PilmisAlban Le Monnier

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