PMID: 2492079Jan 1, 1989Paper

Use of the cytokinesis-block method for the analysis of micronuclei in V79 Chinese hamster lung cells: results with mitomycin C and cyclophosphamide

Mutation Research
G KrishnaJ C Theiss

Abstract

The cytochalasin B (CYB)-blocked binucleated cell assay has been explored to analyze micronuclei and cell cycle kinetics using 2 known mutagenic carcinogens in V79 Chinese hamster lung cells. To determine the optimum time to obtain the maximum number of binucleated cells for micronucleus analysis, duplicate cultures of exponentially growing cells were treated with 3 micrograms/ml CYB for varying durations (8-48 h). A peak appearance of binucleated cells at 16 h in the presence of CYB suggested this as an optimum time for micronucleus analysis in binucleated V79 cells. To evaluate the capacity for induction of micronuclei in V79 cells, 2 mutagenic carcinogens, mitomycin C (0.125-1.0 micrograms/ml) and cyclophosphamide (2-12 micrograms/ml) were tested in duplicate cultures. Mitomycin C, a direct-acting alkylating agent, caused approximately an 18-fold increase in micronucleus frequency over controls at the highest concentration tested (1.0 micrograms/ml), and this increase occurred in a dose-related manner (r = 0.92). The concentrations of mitomycin C tested also caused a significant dose-related cell cycle delay, thus suggesting cytotoxicity to V79 cells. Cyclophosphamide, an indirect-acting alkylating agent, requiring the prese...Continue Reading

References

Jan 1, 1986·Environmental Mutagenesis·E D Thompson
Feb 1, 1985·Mutation Research·M Fenech, A A Morley
Jul 1, 1981·Mutation Research·S A LattS Wolff

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Citations

Jun 3, 2000·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·E Hurná, S Hurná
Jan 1, 1995·Journal of Biomedical Science·M. GabrieleF. Majone
Jul 1, 1997·Environmental Health Perspectives·Y C HongS M Jang
Jan 1, 1991·Teratogenesis, Carcinogenesis, and Mutagenesis·B Z ZhongT M Ong
Jan 29, 2003·Environmental and Molecular Mutagenesis·Sophie MeintièresDaniel Marzin
Jan 17, 1996·Mutation Research·C F Lotfi, G M Machado-Santelli
Aug 17, 1996·Mutation Research·P R Turner, W A Denny

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