PMID: 2494655Mar 1, 1989Paper

Use of the DNA polymerase chain reaction for homology probing: isolation of partial cDNA or genomic clones encoding the iron-sulfur protein of succinate dehydrogenase from several species

Proceedings of the National Academy of Sciences of the United States of America
S J GouldI E Scheffler

Abstract

The DNA polymerase chain reaction was developed for in vitro amplification of specific DNA sequences, and it has been used for a wide variety of purposes in several fields. We have developed an application of the polymerase chain reaction that is useful for the isolation of partial cDNA or genomic clones of conserved genes. We used this technique to clone the gene encoding the iron protein subunit (27 kDa) of succinate dehydrogenase (EC 1.3.5.1) from several species, including human, rat, Drosophila melanogaster, Arabidopsis thaliana, Schizosaccharomyces pombe, and Saccharomyces cerevisiae. Mixed oligonucleotide primers corresponding to two conserved regions of the protein were used in conjunction with genomic and cDNA templates in the reaction. The primers contained all possible nucleotide combinations that could encode the corresponding peptide sequences. These oligonucleotide mixtures contained 262,144 (2(18] and 8192 (2(13] unique sequences, respectively. Use of the polymerase chain reaction for homology probing allows one to utilize more complex mixtures of oligonucleotides as probes than is possible with filter hybridization screening techniques. In addition, the polymerase chain reaction offers the advantage of synthesiz...Continue Reading

References

Jan 1, 1985·Annual Review of Biochemistry·Y Hatefi
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S K Hanks
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·U B Gyllensten, H A Erlich
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N TouchotA Tavitian
Jun 1, 1986·Bioscience Reports·S A KrawetzG H Dixon
Jan 1, 1980·Cytogenetics and Cell Genetics·J T MascarelloI E Scheffler

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Citations

Jan 1, 1996·Archives of Insect Biochemistry and Physiology·R M RoeD M Thompson
Jan 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T FujiiH Westphal
Sep 1, 1993·Somatic Cell and Molecular Genetics·S LeckschatI E Scheffler
Oct 25, 1995·Molecular & General Genetics : MGG·C JonakH Hirt
Apr 1, 1994·Insect Biochemistry and Molecular Biology·J E HendersonD M Soderlund
Apr 6, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·D BarsyteD A Lovejoy
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S SuzukiH Tanihara
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R N Van GelderJ H Eberwine
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L MonacoM Vaughan
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A S KopinA B Leiter
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J ChernoffB G Neel
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B KeithG R Fink
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H HirtD Dudits
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·W SunM Montal
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K ChoiG Keller
Dec 1, 1995·Molecular Biology of the Cell·H FaresJ R Pringle
Aug 25, 1989·Nucleic Acids Research·R Sommer, D Tautz
Dec 27, 1996·Annals of the New York Academy of Sciences·G DodtS J Gould

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