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Use of the Escherichia coli chromosomal DHFR gene as selection marker in mammalian cells

Journal of Biotechnology

Dec 1, 1995

F A Asselbergs, R Widmer

Abstract

The folA gene, the chromosomal dhfr gene of Escherichia coli, was engineered for expression in mammalian cells. In contrast to plasmid-derived bacterial dhfr genes previously used as selection markers in mammalian cells, the folA gene product is inhibitable by methotrexate (MTX) and tri...read more

Mentioned in this Paper

Ovarian Diseases
Flow Cytometry
Chinese Hamster
Shuttle Vectors
Transfection
Dhfr
Alkalescens-Dispar Group
Both Ovaries
Ovarian Neoplasm
Ovary
9
Paper Details
References
  • References18
  • Citations1
12
  • References18
  • Citations1

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Use of the Escherichia coli chromosomal DHFR gene as selection marker in mammalian cells

Journal of Biotechnology

Dec 1, 1995

F A Asselbergs, R Widmer

PMID: 8562018

DOI: 10.1016/0168-1656(95)00131-3

Abstract

The folA gene, the chromosomal dhfr gene of Escherichia coli, was engineered for expression in mammalian cells. In contrast to plasmid-derived bacterial dhfr genes previously used as selection markers in mammalian cells, the folA gene product is inhibitable by methotrexate (MTX) and tri...read more

Mentioned in this Paper

Ovarian Diseases
Flow Cytometry
Chinese Hamster
Shuttle Vectors
Transfection
Dhfr
Alkalescens-Dispar Group
Both Ovaries
Ovarian Neoplasm
Ovary
9

Similar Papers Found In These Feeds

Ovarian Cancer

Ovarian cancers, benign or malignant, originate in cells of the ovaries. They are classified based on their tissue or origin, which includes the totipotent germ cells, epithelium, and the stromal endocrine cells. Here is the latest research.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America

Integrons in Xanthomonas: a source of species genome diversity

Proceedings of the National Academy of Sciences of the United States of AmericaMarch 10, 2005
Michael R GillingsAndrew J Holmes
Paper Details
References
  • References18
  • Citations1
12
  • References18
  • Citations1
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