Use of the fibroblast model in the study of cellular senescence

Methods in Molecular Medicine
V J CristofaloR G Allen

Abstract

In this chapter, we present standard procedures for the culture of human cells that exhibit a finite proliferative capacity (replicative life-span). The use of a cell culture model has the advantage of providing a controlled environment to study a wide variety of cellular phenomena. It also has the inherent limitation of isolating cells from the regulatory elements that might be provided by other types of cells in vivo. Nevertheless, cell culture models have been crucial to our current understanding of mechanisms of growth, differentiation, development, and neoplasia and numerous other disease states. In this chapter we present procedures for human fibroblast culture including serumfree cultivation of cells, which is necessary when the cellular environment must be fully defined. In addition, we present procedures for the determination of replicative life-span, saturation density, and assessment of replicative capacity from labeled thymidine incorporation in fibroblasts. The methods described here have been well tested and provide highly reproducible results (1, 2).

Citations

Feb 12, 2017·Journal of Forensic Sciences·Sophie ColombArnaud Bourdin
Nov 26, 2015·Physiological Reports·Ana Martín-Pardillos, Víctor Sorribas
Aug 16, 2019·Cellular and Molecular Life Sciences : CMLS·Justyna Mikuła-PietrasikKrzysztof Książek
Aug 20, 2018·Bulletin of Experimental Biology and Medicine·D V GrishinN N Sokolov
Jan 13, 2011·Bioelectromagnetics·Katherine SullivanRobert G Allen
Mar 21, 2020·Frontiers in Cell and Developmental Biology·Yoyo Chih-Yun YuShau-Ping Lin

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