Use of the gel agglutination technique for determination of fetomaternal hemorrhage

Transfusion
Abdulgabar SalamaJ W Dudenhausen

Abstract

Adequate administration of Rh immune globulin requires an accurate determination of the number of D-positive cells in the circulation of D-negative women. Although several tests have been described for the detection of fetomaternal hemorrhage, there is still a need for a rapid, simple, and clinically relevant screening test. Serial dilutions of a monoclonal anti-D were incubated with stock solutions (0.2 mL) of adult D-negative red cells in the absence or presence of various amounts of fetal D-positive cells (0.1, 0.2, 0.3, 0.4, and 0.5%). After incubation, the supernatants were tested against D-positive red cells by using the new, gel agglutination technique (GAT). After the GAT was adapted to detect D-positive cells at concentrations of > or = 0.2 percent, unselected postpartum samples from D-negative women (n = 420) who delivered D-positive infants were analyzed by both the new test and the Kleihauer-Betke test (KBT). Three of a total of 420 postpartum samples were positive (> or = 0.4% fetal cells), and 406 were negative in both tests. One had 0.5-percent fetal cells in the KBT and gave negative results in the GAT. The latter test was, however, performed after administration of Rh immune globulin. The KBT gave false-positiv...Continue Reading

Citations

Oct 21, 2006·Current Opinion in Hematology·Morten Hanefeld DziegielAdela Berkowicz
Jan 16, 2007·Forensic Science International : Synergy·S A PadoschB Madea
Jan 30, 1999·Transfusion Medicine Reviews·J K Duguid, I M Bromilow
Mar 31, 2012·Acupuncture in Medicine : Journal of the British Medical Acupuncture Society·Marcio DiasBeni Olej
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Jul 10, 2010·Acupuncture in Medicine : Journal of the British Medical Acupuncture Society·Marcio DiasBeni Olej

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