Use of the glucosyltransferase UGT71B6 to disturb abscisic acid homeostasis in Arabidopsis thaliana

The Plant Journal : for Cell and Molecular Biology
David M PriestD J Bowles

Abstract

A glucosyltransferase (GT) of Arabidopsis, UGT71B6, recognizing the naturally occurring enantiomer of abscisic acid (ABA) in vitro, has been used to disturb ABA homeostasis in planta. Transgenic plants constitutively overexpressing UGT71B6 (71B6-OE) have been analysed for changes in ABA and the related ABA metabolites abscisic acid glucose ester (ABA-GE), phaseic acid (PA), dihydrophaseic acid (DPA), 7'-hydroxyABA and neo-phaseic acid. Overexpression of the GT led to massive accumulation of ABA-GE and reduced levels of the oxidative metabolites PA and DPA, but had marginal effect on levels of free ABA. The control of ABA homeostasis, as reflected in levels of the different metabolites, differed in the 71B6-OEs whether the plants were grown under standard conditions or subjected to wilt stress. The impact of increased glucosylation of ABA on ABA-related phenotypes has also been assessed. Increased glucosylation of ABA led to phenotypic changes in post-germinative growth. The use of two structural analogues of ABA, known to have biological activity but to differ in their capacity to act as substrates for 71B6 in vitro, confirmed that the phenotypic changes arose specifically from the increased glucosylation caused by overexpressi...Continue Reading

References

Jan 1, 1996·Plant Physiology·K M Léon-KloosterzielM Koornneef
Oct 1, 1996·The Plant Journal : for Cell and Molecular Biology·K M Léon-KloosterzielM Koornneef
Jan 1, 1997·The Plant Cell·J C JangJ Sheen
Nov 24, 1999·Trends in Plant Science·A J Cutler, J E Krochko
Oct 24, 2000·The Journal of Biological Chemistry·Y LiD J Bowles
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·M SeoT Koshiba
Nov 7, 2000·Biochemistry·A J CutlerS R Abrams
Feb 22, 2001·Genome Biology·J RossD J Bowles
Apr 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·L Lopez-MolinaN H Chua
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C D Rock, J A Zeevaart
Apr 15, 2003·Plant Physiology·Steven H SchwartzJan A D Zeevaart
Dec 13, 2003·Plant Physiology·Rong ZhouSuzanne R Abrams
Jul 9, 2004·The EMBO Journal·Eng-Kiat Lim, Dianna J Bowles
Nov 25, 2004·Phytochemistry·L Irina ZahariaSuzanne R Abrams
Feb 11, 2005·Journal of the American Chemical Society·James M NyanguluSuzanne R Abrams
Apr 30, 2005·Current Opinion in Plant Biology·Dianna BowlesBrigitte Poppenberger
May 3, 2005·Annual Review of Plant Biology·Eiji Nambara, Annie Marion-Poll
May 1, 1972·Plant Physiology·P E KriedemannA C Leopold
May 4, 2006·Annual Review of Plant Biology·Dianna BowlesFabián E Vaistij
Jan 1, 2002·The Arabidopsis Book·Ruth R Finkelstein, Christopher D Rock
Dec 1, 1984·Journal of Plant Physiology·H Lehmann, H R Schütte

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Citations

Mar 19, 2011·Journal of Plant Research·Mitsunori Seo, Tomokazu Koshiba
Dec 21, 2011·Omics : a Journal of Integrative Biology·Khushwant SinghAshwani Pareek
Feb 3, 2009·Journal of Experimental Botany·Caroline SirichandraJeffrey Leung
Jun 23, 2010·Journal of Experimental Botany·Laura ErnstDaniel P Schachtman
Dec 1, 2011·Science Signaling·Archana Joshi-SahaJeffrey Leung
Jun 15, 2011·Annual Review of Phytopathology·Alexandre Robert-SeilaniantzJonathan D G Jones
Mar 3, 2010·Annual Review of Plant Biology·Tae-Houn KimJulian I Schroeder
Jan 1, 2012·Rice·Nenghui YeJianhua Zhang
Jul 25, 2009·The Plant Journal : for Cell and Molecular Biology·Julia SantiagoPedro L Rodriguez
Apr 25, 2012·Plant Science : an International Journal of Experimental Plant Biology·Kevin N BaronClaudio Stasolla
Mar 19, 2011·Phytochemistry·Masanori OkamotoEiji Nambara
May 25, 2010·Trends in Plant Science·Agepati S RaghavendraErwin Grill
Nov 7, 2015·Plant & Cell Physiology·Matías ManziAurelio Gómez-Cadenas
Dec 10, 2015·Frontiers in Plant Science·Carla A SpenceHarsh P Bais
Mar 17, 2007·Journal of Plant Physiology·Jae Hyung KoJoong-Hoon Ahn
Feb 6, 2008·The Plant Journal : for Cell and Molecular Biology·Akio NoguchiEiichiro Ono
Aug 23, 2007·The Plant Journal : for Cell and Molecular Biology·Alexander ChristmannErwin Grill
Mar 30, 2011·The Plant Journal : for Cell and Molecular Biology·Keiko Yonekura-Sakakibara, Kousuke Hanada
Sep 23, 2009·The Plant Journal : for Cell and Molecular Biology·Izabela SzostkiewiczErwin Grill
Mar 22, 2014·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Masahiro NakagawaToshio Hakoshima
Mar 24, 2016·BMC Plant Biology·Supakan RattanakonGrant R Cramer

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