PMID: 11934487Apr 6, 2002Paper

Use of the gyrB gene for the identification of Pandoraea species

FEMS Microbiology Letters
T Coenye, J J LiPuma

Abstract

The recently described genus Pandoraea consists of five named species and four unnamed genomospecies, several of which have been identified in clinical specimens including respiratory secretions from persons with cystic fibrosis. We investigated whether it is possible to distinguish species of the genus Pandoraea by means of restriction fragment length polymorphism (RFLP) analysis and direct sequencing of the gyrB gene. Sixty-seven Pandoraea isolates were included. Species-specific RFLP patterns were obtained following digestion of the PCR-amplified gyrB gene with MspI. Specificity of RFLP groupings was confirmed by direct sequencing of several representative isolates. Our results indicate that RFLP analysis and sequencing of the gyrB gene are useful for the identification of Pandoraea species. We also found that further taxonomic studies within the beta-Proteobacteria using the gyrB gene would benefit from the development of additional primers allowing more efficient amplification of the gyrB gene. Our data also indicate that the taxonomic status of Pandoraea genomospecies 2 should be reinvestigated.

References

Jan 11, 1992·International Journal of Systematic Bacteriology·G E FoxP Jurtshuk
Oct 23, 1997·International Journal of Systematic Bacteriology·T PalysF M Cohan
Sep 12, 1998·International Journal of Systematic Bacteriology·S Yamamoto, S Harayama
Jan 5, 2000·Journal of Clinical Microbiology·H KasaiS Harayama
May 29, 2000·International Journal of Systematic and Evolutionary Microbiology·H KasaiS Harayama
Nov 29, 2001·Journal of Clinical Microbiology·T CoenyeJ J LiPuma

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Citations

May 8, 2003·Journal of Clinical Microbiology·Martin E StryjewskiBarbara D Alexander
Apr 9, 2010·Clinical Microbiology Reviews·John J Lipuma
Dec 27, 2015·BMC Infectious Diseases·Nicolas DegandJacqueline Carrère
Mar 4, 2008·FEMS Microbiology Letters·Silvia TabacchioniRenato Fani
Oct 1, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·F Bittar, J-M Rolain
Mar 25, 2004·FEMS Immunology and Medical Microbiology·Tom CoenyePeter Vandamme
Jan 25, 2019·International Journal of Systematic and Evolutionary Microbiology·Wah Seng See-TooLouise F Roddam
Oct 16, 2019·Antimicrobial Agents and Chemotherapy·Lindsay J CaverlyJohn J LiPuma
Oct 19, 2010·International Journal of Systematic and Evolutionary Microbiology·Nurettin SahinAbdurrahman U Tamer
Feb 6, 2008·Journal of Basic Microbiology·Héctor Antonio CristóbalCarlos Mauricio Abate
Jun 9, 2016·International Journal of Systematic and Evolutionary Microbiology·Sang Eun JeongChe Ok Jeon
Jun 12, 2014·Sensors·Robson EeKok-Gan Chan
Oct 24, 2017·Frontiers in Microbiology·Chloé DupontHélène Marchandin
Jun 1, 2016·Frontiers in Microbiology·Mark AmbroseLouise F Roddam
Sep 10, 2015·Antimicrobial Agents and Chemotherapy·Ines Schneider, Adolf Bauernfeind
Nov 30, 2019·Frontiers in Microbiology·Charlotte PeetersPeter Vandamme
Oct 24, 2020·International Journal of Systematic and Evolutionary Microbiology·Sabrine DhaouadiAli Rhouma
Jan 15, 2011·Clinical Microbiology Reviews·Alan R HauserSusanna A McColley

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