Use of the mouse ear vesicant model to evaluate the effectiveness of ebselen as a countermeasure to the nitrogen mustard mechlorethamine

Journal of Applied Toxicology : JAT
Anju LullaBlase Billack

Abstract

Previous studies in this and other laboratories have demonstrated that ebselen (EB-1), an organoselenium compound, spares cells from mechlorethamine (HN2) toxicity in vitro. In the present study, the hypothesis that EB-1 will reduce dermal toxicity of HN2 in vivo is put forward and found to have merit. Using the mouse ear vesicant model (MEVM), HN2, applied topically, showed a dose-dependent effect upon ear swelling and thickness 24 h after treatment; whereas tissue injury consistent with vesication was observed at the higher test doses of HN2 (≥ 0.250 µmol per ear). To examine HN2 countermeasure activity using the MEVM, either hydrocortisone (HC), as a positive control, or EB-1, the test countermeasure, was administered as three topical treatments 15 min, 4 and 8 h after HN2 exposure. Using this approach, both HC and EB-1 were found to reduce tissue swelling associated with HN2 toxicity 24 h after exposure to the vesicant. Taken together, these data demonstrate for the first time the effectiveness of EB-1 as a vesicant countermeasure in a relevant in vivo model.

References

Sep 1, 1990·Electroencephalography and Clinical Neurophysiology·B Elie, P Guiheneuc
Feb 1, 1991·Journal of Pharmaceutical Sciences·K SatoY Morimoto
Dec 1, 1971·Archives of Dermatology·W Montagna
Apr 1, 1972·Biulleten' eksperimental'noĭ biologii i meditsiny·A F LeshchinskiĭT S Barkagan
Feb 1, 1997·Journal of Dermatological Science·K J SmithB E Hackley
Nov 5, 1997·The New England Journal of Medicine·S W LambertsF H de Jong
Jan 28, 1998·Stroke; a Journal of Cerebral Circulation·T YamaguchiH Yasuhara
May 29, 1998·Skin Pharmacology and Applied Skin Physiology·G A Simon, H I Maibach
Nov 23, 2000·Current Opinion in Genetics & Development·J L Thorne
Jun 1, 2001·Journal of the Royal Society of Medicine·A M Porter
Feb 20, 2002·Drug and Chemical Toxicology·Somnath SinghJagdish Singh
Mar 31, 2004·Journal of Applied Toxicology : JAT·Shlomit DachirTamar Kadar
Feb 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Lise A Labiche, James C Grotta
Aug 9, 2005·Toxicology·Kai Kehe, Ladislaus Szinicz
Oct 11, 2005·Drug Discovery Today·Eric D Lynch, Jonathan Kil
Nov 29, 2005·Clinical and Experimental Dermatology·R N SaladiA N Persaud
Feb 21, 2006·Journal of Applied Toxicology : JAT·Michael P ShakarjianDonald R Gerecke
Jun 2, 2006·Apoptosis : an International Journal on Programmed Cell Death·Matthieu SourdevalFrancelyne Marano
Jan 25, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Jens EhmckeStefan Schlatt
Sep 4, 2007·Toxicology Letters·Muharrem UcarTurgut Topal
Sep 25, 2007·Advanced Drug Delivery Reviews·Biana Godin, Elka Touitou
Oct 4, 2007·Journal of Biochemical and Molecular Toxicology·Grace ChanBlase Billack
Apr 24, 2008·Toxicology and Industrial Health·Diane Hardej, Blase Billack
Nov 7, 2008·Cutaneous and Ocular Toxicology·Maria A Pino, Blase Billack
Dec 27, 2008·Toxicology Letters·Claudia KaracsonyiElliott Kagan
Jun 22, 2010·Inhalation Toxicology·Mostafa GhaneiZohreh Poursaleh
Oct 5, 2010·Toxicology and Applied Pharmacology·Vasanthi R SunilDebra L Laskin
Oct 12, 2010·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Marion K GordonPatrick J Sinko
Feb 9, 2011·Neurotoxicology·Zhaobao YinMichael Aschner
Dec 3, 2011·Veterinary Pathology·D K TaylorJ P Sundberg
Jan 10, 2013·Nature Communications·Nisha SinghGrant C Churchill
May 8, 2013·Journal of Biochemical and Molecular Toxicology·Anju LullaBlase Billack

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Citations

Aug 10, 2016·Annals of the New York Academy of Sciences·Carl J LaceyNed D Heindel
Sep 27, 2018·Drug and Chemical Toxicology·Hemanta C Rao TumuBlase Billack
Nov 26, 2019·Interdisciplinary Toxicology·Benedette J CuffariBlase Billack

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