PMID: 20650061Feb 1, 1995Paper

Use of the MTT assay for estimating toxicity in primary astrocyte and C6 glioma cell cultures

Toxicology in Vitro : an International Journal Published in Association with BIBRA
M R CooksonV W Pentreath

Abstract

Primary cultures of rat cortical astrocytes were exposed to 25 neurotoxic and non-neurotoxic compounds for 24 hr over a concentration range of 0.001 to 1000 mug/ml and the effects were quantified using the MTT assay. EC(50) values were obtained for nine of the compounds in the tested range, while increases in MTT conversion were seen at sub-cytotoxic concentrations for 12 compounds. The concentrations causing activation of this system were found, with some exceptions (notably organotin compounds) to be similar to those previously reported to cause increases in expression of the astrocyte marker glial fibrillary acidic protein. MTT conversion was also measured in the C6 cell line and, with some exceptions, the response to toxicants was found to be the same as that in the primary cultures of astrocytes. However, this was only the case when the C6 cells were pretreated for 48 hr with 0.5 mM dibutyryl cyclic AMP. This study represents the first direct comparison between primary astrocytes and C6 cells using a broad range of chemical neurotoxicants.

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J LaterraG W Goldstein
Jan 1, 1991·Archives of Toxicology·A BruininkF Riesen
Jan 1, 1986·Brain Research·D L Davies, A Vernadakis
Aug 1, 1982·Journal of Neurochemistry·E T Browning, W J Nicklas
Nov 1, 1984·Journal of Neurochemistry·S KumarJ de Vellis
Apr 1, 1994·Trends in Neurosciences·M K McMillianK R Pennypacker
Apr 1, 1993·Mutation Research·I D AdlerE Schmid
Jun 7, 2005·Expert Opinion on Drug Safety·James P O'Callaghan, Krishnan Sriram
Jun 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M R Cookson, V W Pentreath
May 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H B JohnstonC K Atterwill
Sep 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C K AtterwillS Williams

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Citations

Mar 30, 2007·Journal of Neural Transmission·J C FitzgeraldE E Billett
Jun 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M R Cookson, V W Pentreath
Apr 12, 2000·Toxicon : Official Journal of the International Society on Toxinology·N RakbaG Lescoat
Aug 26, 2003·Brain Research. Developmental Brain Research·Claudia RöhlJobst Sievers
Jun 15, 2000·Journal of Neurochemistry·L A De GirolamoA J Hargreaves
Sep 29, 2015·Experimental Neurobiology·Hwa Yeon JoSun Ha Paek
Jan 1, 2004·Nonlinearity in Biology, Toxicology, Medicine·V W Pentreath, C Mead
Mar 10, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Lanhai LüJie Xu
Nov 7, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Delfina M RomeroMónica L Kotler
Apr 25, 2008·Critical Reviews in Toxicology·Edward J Calabrese
Jun 24, 2008·Critical Reviews in Toxicology·Edward J Calabrese
Jan 1, 2005·Nonlinearity in Biology, Toxicology, Medicine·N Debbie SlamonVic W Pentreath
Feb 24, 2001·Human & Experimental Toxicology·V W Pentreath, N D Slamon
Apr 16, 1998·Environmental Health Perspectives·G J HarryH A Tilson
Oct 12, 2001·American Journal of Physiology. Endocrinology and Metabolism·A G TrentinV Moura Neto
Apr 3, 2020·Metallomics : Integrated Biometal Science·Samantha E HuffmanMichael I Webb
Feb 7, 2001·Journal of Neurochemistry·L A De GirolamoE E Billett
Mar 22, 2016·Experimental and Therapeutic Medicine·Zhong Wang, Qianxue Chen
Feb 7, 2021·Applied Microbiology and Biotechnology·María Del Milagro TeranJean Guy LeBlanc

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