Use of the QIAGEN GeneReader NGS system for detection of KRAS mutations, validated by the QIAGEN Therascreen PCR kit and alternative NGS platform

BMC Cancer
Agus DarwantoThomas Rothmann

Abstract

The detection of somatic mutations in primary tumors is critical for the understanding of cancer evolution and targeting therapy. Multiple technologies have been developed to enable the detection of such mutations. Next generation sequencing (NGS) is a new platform that is gradually becoming the technology of choice for genotyping cancer samples, owing to its ability to simultaneously interrogate many genomic loci at massively high efficiency and increasingly lower cost. However, multiple barriers still exist for its broader adoption in clinical research practice, such as fragmented workflow and complex bioinformatics analysis and interpretation. We performed validation of the QIAGEN GeneReader NGS System using the QIAact Actionable Insights Tumor Panel, focusing on clinically meaningful mutations by using DNA extracted from formalin-fixed paraffin-embedded (FFPE) colorectal tissue with known KRAS mutations. The performance of the GeneReader was evaluated and compared to data generated from alternative technologies (PCR and pyrosequencing) as well as an alternative NGS platform. The results were further confirmed with Sanger sequencing. The data generated from the GeneReader achieved 100% concordance with reference technologies...Continue Reading

References

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Citations

Dec 10, 2017·Journal of Translational Medicine·Filippo Del VecchioAlessandra Tessitore
Jul 23, 2020·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·William Mathieson, Geraldine A Thomas
May 16, 2018·Academic Pathology·Yuriy O AlekseyevDaniel Remick
May 2, 2020·World Journal of Clinical Oncology·Jorge Gómez, Panagiotis Tsagozis
Oct 12, 2018·Virchows Archiv : an International Journal of Pathology·Giulio RivaMatteo Brunelli
Jul 16, 2021·Critical Reviews in Biochemistry and Molecular Biology·Jörn GlöklerMarcus Frohme

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Methods Mentioned

BETA
PCR
Assay
deamination

Software Mentioned

AcroMetrix
QCI Analyze
QIAact
AcroMetrix Oncology Hotspot
GATK
Interpret
QIAGEN GeneReader NGS System
QIAGEN
GeneReader NGS System
GeneReader System

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