Use of the rat forelimb compression model to create discrete levels of bone damage in vivo

Journal of Biomechanics
Brian A Uthgenannt, Matthew J Silva

Abstract

Skeletal responses to damage are significant for understanding the etiology of stress fractures and possibly osteoporotic fractures. We refined the rat forelimb-loading model to produce a range of sub-fracture damage levels during in vivo cyclic loading. A total of 98 right forelimbs of anesthetized, male, 5-month old Fischer rats were loaded cyclically (2 Hz) in axial compression. Rats were killed immediately after loading. In the first experiment, forelimbs were loaded to fracture, which occurred after an increase in peak displacement of 2.0+/-0.2 mm, independent of peak force or cycle number. In the next experiment, we loaded forelimbs at a constant peak force until the peak displacement increased by 0.6-1.8 mm (30-90% of fracture displacement). Mechanical properties of the loaded (right) and contralateral control (left) ulnae were determined ex vivo using three-point bending, and cracks were analyzed using micro-computed tomography. Results demonstrated a dose-response between increased forelimb displacement and increased ulnar damage, with four discrete damage levels. "Low" damage was produced by cyclic loading to 30% of fracture displacement, with no visible cracks and a 10% strength loss. "Mild" damage was produced by lo...Continue Reading

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Citations

Jun 7, 2007·Calcified Tissue International·Hironori MatsuzakiMatthew J Silva
Jun 21, 2011·Biomechanics and Modeling in Mechanobiology·Antonia TorcasioG Harry van Lenthe
Sep 10, 2011·Medical Engineering & Physics·Yunkai LuMark L Johnson
May 28, 2011·Journal of the Mechanical Behavior of Biomedical Materials·J MacioneS P Kotha
Jun 20, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Brian A UthgenanntMatthew J Silva
Apr 13, 2011·Arthritis Care & Research·Shawn M RobbinsMonica R Maly
Aug 1, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Lisa J KiddMark R Forwood
Jun 10, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Mary L BouxseinRalph Müller
May 21, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·L J KiddM R Forwood
Mar 20, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ryan E TomlinsonMatthew J Silva
Sep 10, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ganesh ThiagarajanMark L Johnson
Oct 10, 2012·Bone·Ryan E TomlinsonMatthew J Silva
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Sep 1, 2020·Bone·M P Akhter, R R Recker
May 20, 2021·Journal of Biomechanics·Chenxi YanMariana E Kersh

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